Characterization of conserved T- and B-cell epitopes in plasmodium falciparum major merozoite surface protein 1

dc.contributor.authorParra, M.
dc.contributor.authorHui, G.
dc.contributor.authorJohnson, A.H.
dc.contributor.authorBerzofsky, J.A.
dc.contributor.authorRoberts, T.
dc.contributor.authorQuakyi, I.A.
dc.contributor.authorTaylor, D.W.
dc.date.accessioned2013-06-21T12:57:22Z
dc.date.accessioned2017-10-16T12:25:39Z
dc.date.available2013-06-21T12:57:22Z
dc.date.available2017-10-16T12:25:39Z
dc.date.issued2000
dc.description.abstractVaccines for P. falciparum will need to contain both T, and B-cell epitopes. Conserved epitopes are the most desirable, but they are often poorly immunogenic. The major merozoite surface protein 1 (MSP-1) is currently a leading vaccine candidate antigen. In this study, six peptides from conserved or partly conserved regions of MSP-1 were evaluated for immunogenicity in B10 congenic mice. Following immunization with the peptides, murine T cells were tested for the ability to proliferate in vitro and antibody responses to MSP-1 were evaluated in vivo. The results showed that one highly conserved sequence (MSP-11, VTHESYQELVKKLEALEDAV; located at amino acid positions 20 to 39) and one partly conserved sequence (MSP-123, GLFHKEKMIL NEEEITTKGA; located at positions 44 to 63) contained both T- and B-cell epitopes. Immunization of mice with these peptides resulted in T-cell proliferation and enhanced production of antibody to MSP-1 upon exposure to merozoites. MSP-11 stimulated T-cell responses in three of the six strains of mice evaluated, whereas MSP-123 was immunogenic in only one strain. Immunization with the other four peptides resulted in T-cell responses to the peptides, but none of the resulting peptide-specific T cells recognized native MSP-1. These results demonstrate that two sequences located in the N terminus of MSP-1 can induce T- and B-cell responses following immunization in a murine model. Clearly, these sequences merit further consideration for inclusion in a vaccine for malaria.en_US
dc.identifier.citationParra, M., Hui, G., Johnson, A. H., Berzofsky, J. A., Roberts, T., Quakyi, I. A., & Taylor, D. W. (2000). Characterization of conserved T- and B-cell epitopes in plasmodium falciparum major merozoite surface protein 1. Infection and Immunity, 68(5), 2685-2691.en_US
dc.identifier.issn00199567
dc.identifier.urihttp://197.255.68.203/handle/123456789/3813
dc.language.isoenen_US
dc.publisherInfection and Immunityen_US
dc.subjectEMTREE drug terms: amino acid; antibody; epitope; merozoite surface protein 1; vaccineen_US
dc.subjectEMTREE medical terms: animal cell; antibody production; antibody response; article; B lymphocyte; enzyme linked immunosorbent assay; immunization; immunogenicity; lymphocyte proliferation; mouse; nonhuman; Plasmodium falciparum; priority journal; T lymphocyteen_US
dc.subjectMeSH: Amino Acid Sequence; Animals; Antibodies, Protozoan; Cell Division; Conserved Sequence; Epitope Mapping; Epitopes, B-Lymphocyte; Epitopes, T-Lymphocyte; Female; H-2 Antigens; Humans; Merozoite Surface Protein 1; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Sequence Data; Peptides; Plasmodium falciparum; Protozoan Vaccines; T-Lymphocytes; Vaccination; Vaccines, Syntheticen_US
dc.titleCharacterization of conserved T- and B-cell epitopes in plasmodium falciparum major merozoite surface protein 1en_US
dc.typeArticleen_US

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