Full genomic characterization of a porcine rotavirus strain detected in an asymptomatic piglet in Accra, Ghana

dc.contributor.authorQuaye, O.
dc.contributor.authorStubbs, S.C.B.
dc.contributor.authorAcquah, M.E.
dc.contributor.authorAdadey, S.M.
dc.contributor.authorKean, I.R.L
dc.contributor.authorGupta, S.
dc.contributor.authorBlacklaws, B.A.
dc.date.accessioned2020-01-23T10:12:54Z
dc.date.available2020-01-23T10:12:54Z
dc.date.issued2020-01-10
dc.descriptionResearch Articleen_US
dc.description.abstractBackground: The introduction of rotavirus A vaccination across the developing world has not proved to be as efficacious as first hoped. One cause of vaccine failure may be infection by zoonotic rotaviruses that are very variable antigenically from the vaccine strain. However, there is a lack of genomic information about the circulating rotavirus A strains in farm animals in the developing world that may be a source of infection for humans. We therefore screened farms close to Accra, Ghana for animals sub-clinically infected with rotavirus A and then sequenced the virus found in one of these samples. Results: 6.1% of clinically normal cows and pigs tested were found to be Rotavirus A virus antigen positive in the faeces. A subset of these (33.3%) were also positive for virus RNA. The most consistently positive pig sample was taken forward for metagenomic sequencing. This gave full sequence for all open reading frames except segment 5 (NSP1), which is missing a single base at the 5′ end. The virus infecting this pig had genome constellation G5-P[7]-I5-R1-C1-M1- A8-N1-T7-E1-H1, a known porcine genotype constellation. Conclusions: Farm animals carry rotavirus A infection sub-clinically at low frequency. Although the rotavirus A genotype discovered here has a pig-like genome constellation, a number of the segments most closely resembled those isolated from humans in suspected cases of zoonotic transmission. Therefore, such viruses may be a source of variable gene segments for re-assortment with other viruses to cause vaccine breakdown. It is recommended that further human and pig strains are characterized in West Africa, to better understand this dynamic.en_US
dc.description.sponsorshipCambridge-Africa Alborada Research Fund through a CAPREX Fellowship to Dr. Osbourne Quaye.en_US
dc.identifier.otherhttps://doi.org/10.1186/s12917-019-2226-9
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/34618
dc.language.isoenen_US
dc.publisherBMC Veterinary Researchen_US
dc.relation.ispartofseries16;11
dc.subjectRotavirus Aen_US
dc.subjectFull genome sequence constellationen_US
dc.subjectPigen_US
dc.subjectPorcineen_US
dc.subjectGhanaen_US
dc.titleFull genomic characterization of a porcine rotavirus strain detected in an asymptomatic piglet in Accra, Ghanaen_US
dc.typeArticleen_US

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