Plasmodium falciparum Kelch Propeller Polymorphisms in Clinical Isolates from Ghana from 2007 to 2016

dc.contributor.authorMatrevi, S.A.
dc.contributor.authorOpoku-Agyeman, P.
dc.contributor.authorQuashie, N.B.
dc.contributor.authorBruku, S.
dc.contributor.authorAbuaku, B.
dc.contributor.authorKoram, K.A.
dc.contributor.authorFox, A.
dc.contributor.authorLetizia, A.
dc.contributor.authorDuah-Quashie, N.O.
dc.date.accessioned2019-12-02T15:38:16Z
dc.date.available2019-12-02T15:38:16Z
dc.date.issued2019-10-22
dc.descriptionResearch Articleen_US
dc.description.abstractThe continuous surveillance of polymorphisms in the kelch propeller domain of Plasmodium falciparum from Africa is important for the discovery of the actual markers of artemisinin resistance in the region. The information on the markers is crucial for control strategies involving chemotherapy and chemoprophylaxis for residents and nonimmune travelers to the country. Polymorphisms in the kelch propeller domain of Ghanaian malaria parasites from three different ecological zones at several time periods were assessed. A total of 854 archived samples (2007 to 2016) collected from uncomplicated malaria patients aged 9 years old from 10 sentinel sites were used. Eighty-four percent had wild-type sequences (PF3D7_1343700), while many of the mutants had mostly nonsynonymous mutations clustered around codons 404 to 650. Variants with different amino acid changes of the codons associated with artemisinin (ART) resistance validated markers were observed in Ghanaian isolates: frequencies for I543I, I543S, I543V, R561P, R561R, and C580V were 0.12% each and 0.6% for R539I. Mutations reported from African parasites, A578S (0.23%) and Q613L (0.23%), were also observed. Three persisting nonsynonymous (NS) mutations, N599Y (0.005%), K607E (0.004%), and V637G (0.004%), were observed in 3 of the 5 time periods nationally. The presence of variants of the validated markers of artemisinin resistance as well as persisting polymorphisms after 14 years of artemisinin-based combination therapy use argues for continuous surveillance of the markers. The molecular markers of artemisinin resistance and the observed variants will be monitored subsequently as part of ongoing surveillance of antimalarial drug efficacy/resistance studies in the country.en_US
dc.description.sponsorshipThe Armed Forces Health Surveillance Branch (AFHSB) and its Global Emerging Infections Surveillance and Response section (GEIS). The field work for the collection of the samples was partly funded by GEIS and the Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM)/National Malaria Control Programme (NMCP; Ghana).en_US
dc.identifier.citationMatrevi SA, Opoku-Agyeman P, Quashie NB, Bruku S, Abuaku B, Koram KA, Fox A, Letizia A, Duah-Quashie NO. 2019. Plasmodium falciparum kelch propeller polymorphisms in clinical isolates from Ghana from 2007 to 2016. Antimicrob Agents Chemother 63:e00802-19. https://doi.org/10 .1128/AAC.00802-19.en_US
dc.identifier.otherhttps://doi.org/10 .1128/AAC.00802-19.
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/33952
dc.language.isoenen_US
dc.publisherEPIDEMIOLOGY AND SURVEILLANCEen_US
dc.relation.ispartofseries63;11
dc.subjectPlasmodium falciparum kelch propeller domain gene (pfk13)en_US
dc.subjectsingle nucleotide polymorphisms (SNPs)en_US
dc.subjectantimalarial drug resistanceen_US
dc.subjectartemisinin (ART)en_US
dc.subjectartemisinin-based combination therapy (ACT)en_US
dc.subjectmalariaen_US
dc.subjectGhanaen_US
dc.titlePlasmodium falciparum Kelch Propeller Polymorphisms in Clinical Isolates from Ghana from 2007 to 2016en_US
dc.typeArticleen_US

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