Genetic diversity of polymorphic vaccine candidate antigens (apical membrane antigen-1, merozoite surface protein-3, and erythrocyte binding antigen-175) in Plasmodium falciparum isolates from Western and Central Africa

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dc.contributor.authorSoulama, I.
dc.contributor.authorBigoga, J.D.
dc.contributor.authorNdiaye, M.
dc.contributor.authorBougouma, E.C.
dc.contributor.authorQuagraine, J.
dc.contributor.authorCasimiro, P.N.
dc.contributor.authorStedman, T.T.
dc.contributor.authorSirima, S.B.
dc.date.accessioned2019-05-03T10:58:35Z
dc.date.available2019-05-03T10:58:35Z
dc.date.issued2011-02
dc.description.abstractThe malaria vaccine candidate antigens erythrocyte binding antigen 175 (EBA-175), merozoite surface protein 3 (MSP-3), and apical membrane antigen (AMA-1) from Plasmodium falciparum isolates from countries in central and west Africa were assessed for allelic diversity. Samples were collected on filter paper from 600 P. falciparum-infected symptomatic patients in Cameroon, Republic of Congo, Burkina Faso, Ghana, and Senegal and screened for classspecific amplification fragments. Genetic diversity, assessed by mean heterozygosity, was comparable among countries. We detected a clinical increase in eba 175 F-allele frequency from west to east across the study region. No statistical difference in msp-3 allele distribution between countries was observed. The ama-1 3D7 alleles were present at a lower frequency in central Africa than in West Africa. We also detected little to no genetic differentiation among sampling locations. This finding indicates that, at least at the level of resolution offered by restriction fragment length polymorphism analysis, these antigens showed remarkable genetic homogeneity throughout the region sampled, perhaps caused by balancing selection to maintain a diverse array of antigen haplotyes. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.en_US
dc.identifier.otherdoi: 10.4269/ajtmh.2011.10-0365
dc.identifier.otherVol.84(2): pp 276-84
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/29763
dc.language.isoenen_US
dc.publisherAmerican Journal of Tropical Medicine and Hygieneen_US
dc.titleGenetic diversity of polymorphic vaccine candidate antigens (apical membrane antigen-1, merozoite surface protein-3, and erythrocyte binding antigen-175) in Plasmodium falciparum isolates from Western and Central Africaen_US
dc.typeArticleen_US

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