Serological Diagnostic Survey and Farmer Perception of Cucumber Mosaic Virus Disease in the Greater Accra Region of Ghana

Abstract

Vegetables are important in diets of practically every household in Ghana. Vegetables are essential dietary portion that provide important vitamins and minerals. In addition to providing farmers with a source of income, vegetable cultivation helps the economy of the country to grow by creating jobs and bringing in substantial amount of foreign currency. Viral diseases are one of the largest obstacles to vegetable production, and in Ghana they are regarded to be a significant factor limiting the output of vegetable production. The Cucumber Mosaic Virus (CMV), one of these viruses, is extremely devastating and infects more plant families than any other plant virus. Unfortunately, since its discovery in 1974, the host range of CMV among important vegetable crops in Ghana has gotten comparatively little research attention. Therefore, the aim of this study was to identify the prevalence, host range, and severity of CMV among the main vegetables grown in Ghana's Greater Accra region, namely in the districts of Tema West, Ga East, and Ayawaso West. A standardized questionnaire was used to conduct a survey involving 120 farmers in these districts to assess the perception of the importance of CMV in vegetable production. It was discovered that the majority of farmers had little to no knowledge about viruses and instead implicated abiotic and biotic factors for their problems. After making extensive visual observations, it was discovered that CMV symptoms were present in every farm that was visited. The presence of CMV in tomato, broccoli, lettuce, spinach, cucumber, radish, and cabbage was confirmed by Enzyme-Linked Immunosorbent Assay (ELISA) on samples taken from symptomatic plants. This is the first account of broccoli, cabbage, lettuce, spinach, and radish in Ghana testing positive for CMV. The disease is spread mechanically through sap inoculation from an infected plant to a healthy plant, according to an ELISA confirmation test. The findings of this study will contribute to the development of efficient control methods that would help manage the disease, particularly given the new host range of CMV discovered in Ghana.