Antiproliferative and Pro-Apoptotic Activity of Diarylheptanoids Isolated from the Bark of Alnus japonica in Human Leukemia Cell Lines

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dc.contributor.authorUto, T.
dc.contributor.authorTung, N.H.
dc.contributor.authorAppiah-Opong, R.
dc.contributor.authorAning, A.
dc.contributor.authorMorinaga, O.
dc.contributor.authorEdoh, D.
dc.contributor.authorNyarko, A.K.
dc.contributor.authorShoyama, Y.
dc.date.accessioned2018-09-13T09:32:28Z
dc.date.available2018-09-13T09:32:28Z
dc.date.issued2015-08
dc.description.abstractAlnus japonica Steud is a tree that grows in damp areas of mountain valleys and has been used as a traditional medicine in Asia. We investigated the antiproliferative activity of hirsutanone (Hir) and oregonin (Ore) in human cancer cell lines and elucidated their mechanisms of action. A cytotoxicity study using a panel of 12 human cancer and 4 normal cell lines indicated that Hir exhibited potent antiproliferative activity against 4 leukemia (Jurkat, U937, THP-1, and HL-60) and 2 colon cancer cell lines (HCT-15 and Colo205). Although Ore suppressed the cell growth of Jurkat and THP-1, its inhibitory potency was weaker than that of Hir. The IC50 values of Hir and Ore in Jurkat were 11.37 μM and 22.16 μM, respectively. Further analysis on Jurkat cells demonstrated that Hir caused a sequence of events involved in apoptosis, including nuclear morphological changes and accumulation of cells with sub-G1 DNA content. Hir led to the cleavage of poly(ADP-ribose) polymerase (PARP) and activation of caspase-3, -8, and -9. In addition, Hir-induced PARP cleavage was completely abolished by specific inhibitors to these caspases. Our data suggested that Hir is a potent antiproliferative compound against the 4 leukemia cell lines and the 2 colon cancer cell lines tested. Furthermore, Hir exerts antiproliferative actions via caspase-dependent apoptotic cell death.en_US
dc.identifier.otherVol. 43, No. 04, pp. 757-767
dc.identifier.otherhttps://doi.org/10.1142/S0192415X15500470
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/24116
dc.language.isoenen_US
dc.publisherThe American Journal of Chinese Medicineen_US
dc.subjectAlnus japonicaen_US
dc.subjectHirsutanoneen_US
dc.subjectOregoninen_US
dc.subjectDiarylheptanoiden_US
dc.subjectAntiproliferationen_US
dc.subjectApoptosisen_US
dc.subjectLeukemia Cellsen_US
dc.titleAntiproliferative and Pro-Apoptotic Activity of Diarylheptanoids Isolated from the Bark of Alnus japonica in Human Leukemia Cell Linesen_US
dc.typeArticleen_US

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