Occurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghana

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dc.contributor.authorBentil, R.E.
dc.contributor.authorAddo, S.O.
dc.contributor.authorBaako, B.O.A.
dc.contributor.authoret al.
dc.date.accessioned2023-07-07T10:20:43Z
dc.date.available2023-07-07T10:20:43Z
dc.date.issued2023
dc.descriptionResearch Articleen_US
dc.description.abstractAbstract Ticks are arthropods of veterinary and medical importance which spread zoonotic pathogens that link animal and human health. In this study, ticks were collected from 448 livestock between February and December 2020 in the Kassena-Nankana Districts of Ghana and screened for the presence of zoonotic pathogens DNA using PCR and sequencing approaches. In total, 1550 ticks were collected and morphologically identified. Three tick genera were identified with Amblyomma variegatum (63%) as the predominant tick species collected. DNA was extracted from 491 tick pools and screened for the presence of DNA of Rickettsia spp. based on the 115 bp fragment of the 17 kDa surface protein and 639 bp of the Outer membrane protein A (ompA) gene and the 295 bp fragment of the transposase gene of Coxiella burnetii IS1111a element. From the 491 pools screened, the DNA of Rickettsia spp. and C. burnetii was detected in 56.8 and 3.7%, respectively. Coinfections were identified in 2.4% of the tick pools. Characterization of the Rickettsia spp. in this study based on the ompA gene showed that the DNA of Rickettsia africae and Rickettsia aeschlimannii accounted for 39.7 and 14.7%, respectively, and were 100% similar to sequences in GenBank. Most R. africae and C. burnetii infections occurred in ticks collected in the wet season, whereas R. aeschlimannii occurred mostly in the dry season. These pathogens are potential public health threats, thus there is a need to implement control measures to reduce the risk of infections in vulnerable populations.en_US
dc.description.sponsorshipThe Uniformed Services University Center for Global Health Engagement (CGHE) through the Global Health Engagement Research Initiative (Grant number: GRANT12767296).en_US
dc.identifier.citationAddo, S.O., Bentil, R.E., Baako, B.O.A. et al. Occurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghana. Exp Appl Acarol 90, 137–153 (2023).en_US
dc.identifier.otherhttps://doi.org/10.1007/s10493-023-00808-0
dc.identifier.urihttp://ugspace.ug.edu.gh:8080/handle/123456789/39521
dc.language.isoenen_US
dc.publisherSpringer Science and Businessen_US
dc.subjectIxodidaeen_US
dc.subjectRickettsia africaeen_US
dc.subjectRickettsia aeschlimanniien_US
dc.subjectCoxiella burnetiien_US
dc.subjectPCRen_US
dc.subjectGhanaen_US
dc.titleOccurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghanaen_US
dc.typeArticleen_US

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