Identification of a fall armyworm (Spodoptera frugiperda)‑specific gene and development of a rapid and sensitive loop‑mediated isothermal amplification assay
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
nature
Abstract
The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread
throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous
migratory pest that can travel over long distances using seasonal winds or typhoons because of its
excellent flying ability, causing serious damage to many crops. For effective FAW control, accurate
species identification is essential at the beginning of the invasion. In this study, the FAW-specific
gene Sf00067 was discovered by performing bioinformatics to develop a fast and accurate tool
for the species-specific diagnosis of this pest. An Sf00067 loop-mediated isothermal amplification
(LAMP) assay was developed, and optimal conditions were established. The Sf00067 6 primer LAMP
(Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of
FAW captured in Korea and FAWs collected from Benin, Africa. Our FAW diagnostic protocol can be
completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar
larva to species determination.
Description
Research Article