A shared Asian origin of the triple-mutant dhfr allele in Plasmodium falciparum from sites across Africa
| dc.contributor.author | Maïga, O. | |
| dc.contributor.author | Djimdé, A.A. | |
| dc.contributor.author | Hubert, V. | |
| dc.contributor.author | Renard, E. | |
| dc.contributor.author | Aubouy, A. | |
| dc.contributor.author | Kironde, F. | |
| dc.contributor.author | Nsimba, B. | |
| dc.contributor.author | Koram, K.A. | |
| dc.contributor.author | Doumbo, O.K. | |
| dc.contributor.author | Le Bras, J. | |
| dc.contributor.author | Clain, J. | |
| dc.date.accessioned | 2012-05-03T16:39:24Z | |
| dc.date.accessioned | 2017-10-16T13:09:38Z | |
| dc.date.available | 2012-05-03T16:39:24Z | |
| dc.date.available | 2017-10-16T13:09:38Z | |
| dc.date.issued | 2007 | |
| dc.description.abstract | Usefulness of sulfadoxine-pyrimethamine as first-line therapy for uncomplicated Plasmodium falciparum malaria and intermittent preventive treatment in pregnancy throughout sub-Saharan Africa is compromised by the spread of dhfr alleles associated with pyrimethamine resistance. A predominant haplotype associated with the N51I+C59R+S108N triple-mutant dhfr allele has been reported recently in 4 African countries. A more comprehensive picture of the evolution of this mutant allele in Africa is lacking. METHODS: Seventy-five P. falciparum isolates carrying the wild-type dhfr allele and 204 carrying the triple-mutant dhfr allele from 11 African countries were selected. The genetic diversity of the chromosomes bearing these alleles was analyzed with 4 microsatellite markers closely linked to the dhfr gene. RESULTS: Seventy-three different 4-locus haplotypes carrying the wild-type dhfr allele were found. By contrast, 175 (85%) of 204 isolates carrying the triple-mutant dhfr allele shared a unique haplotype, identical to the one identified in Thailand. For the remaining triple-mutant isolates and one isolate with the quadruple-mutant dhfr allele N51I+C59R+S108N+I164L), haplotypes were closely related to the predominant haplotype by mutation or recombination. CONCLUSIONS: Migration of parasites carrying an ancestral triple-mutant dhfr allele drives the spread of dhfr alleles associated with pyrimethamine resistance throughout West and Central Africa. | en_US |
| dc.identifier.uri | http://197.255.68.203/handle/123456789/1032 | |
| dc.language.iso | en | en_US |
| dc.publisher | Journal of Infectious Diseases 196(1):165-72 | en_US |
| dc.title | A shared Asian origin of the triple-mutant dhfr allele in Plasmodium falciparum from sites across Africa | en_US |
| dc.type | Article | en_US |