Structural Elucidation and Antiplasmodial Activities of Some Compounds Isolated from The Rhizome of Cochlospermum Tinctorium
Date
2001-10
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Publisher
University of Ghana
Abstract
Cochlospernum tinctorium A. Rich was investigated for its chemical constituents and its
antiplasmodial activities. The chemical constituents of petroleum ether, dichloromethane
and ethyl acetate extracts of the rhizome of the plant were successively studied. The in
vitro antiplasmodial activities for petroleum ether, dichloromethane, ethyl acetate,
ethanol and water extracts as well as the isolated compounds were determined.
Three compounds isolated from the dichloromethane extract have been identified by
spectroscopic methods to be triacontanol, triacontanyl p-coumarate and triacontanyl
ferulate. This is the first report of the isolation of the two esters and the long chain
saturated alcohol from the plant. Another compound was obtained from the
dichloromethane extract and coded D9.
The petroleum ether extract of the rhizome yielded stigmasterol and two compounds
which were coded PE6 and PE11. The ethyl acetate extract of the rhizome yielded two
compounds which were coded E13 and E166
Using an in vitro technique, the inhibition of growth of Plasmodium falciparum
(chloroquine sensitive, 3D7 and chloroquine resistance DD2, strains,) were investigated
based on the principle of uptake of 3H-hypoxanthine by viable parasites on various
extracts of Cochlospermum tinctorium and also on the isolated compounds with
chloroquine as standard.
The percentage inhibition for all the extracts and isolated compounds were found to be
dose-dependant, with the aqueous and dichloromethane extracts being more active with
IC50 values 32.27 (ig/ml and 24.34 |J.g/ml for the chloroqiune sensitive and 30.90 jig/ml
and 23.45 |j.g/m! for the chloroquine resistant strains respectively.
Description
Thesis (MSc.) - University of Ghana, 2001