Dynamics of RecA-mediated repair of replication-dependent DNA breaks

dc.contributor.authorAmarh, V.
dc.contributor.authorWhite, M.A.
dc.contributor.authorLeach, D.R.F.
dc.date.accessioned2019-07-04T09:31:03Z
dc.date.available2019-07-04T09:31:03Z
dc.date.issued2018-05
dc.description.abstractChromosomal replication is the major source of spontaneous DNA double-strand breaks (DSBs) in living cells. Repair of these DSBs is essential for cell viability, and accuracy of repair is critical to avoid chromosomal rearrangements. Repair of replication-dependent DSBs occurs primarily by homologous recombination with a sister chromosome. However, this reaction has never been visualized at a defined chromosomal locus, so little is known about its spatial or temporal dynamics. Repair of a replication-independent DSB generated in Escherichia coli by a rare-cutting endonuclease leads to the formation of a bundle of RecA filaments. In this study, we show that in contrast, repair of a replication-dependent DSB involves a transient RecA focus localized in the central region of the cell in which the DNA is replicated. The recombining loci remain centrally located with restricted movement before segregating with little extension to the period of postreplicative sister-chromosome cohesion. The spatial and temporal efficiency of this reaction is remarkable.en_US
dc.identifier.otherDOI: 10.1083/jcb.201803020
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/31238
dc.language.isoenen_US
dc.publisherJournal of Cell Biologyen_US
dc.titleDynamics of RecA-mediated repair of replication-dependent DNA breaksen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dynamics of RecA-mediated repair of replication-dependent DNA breaks.pdf
Size:
1.74 MB
Format:
Adobe Portable Document Format
Description:

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description: