Diversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markers

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dc.contributor.authorEgbadzor, K.F.
dc.contributor.authorOfori, K.
dc.contributor.authorYeboah, M.
dc.contributor.authorAboagye, L.M.
dc.contributor.authorOpoku-Agyeman, M.O.
dc.contributor.authorDanquah, E.Y.
dc.contributor.authorOffei, S.K.
dc.date.accessioned2018-11-19T11:19:37Z
dc.date.available2018-11-19T11:19:37Z
dc.date.issued2014-09
dc.description.abstractSingle Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection. © 2014, Egbadzor et al.; licensee Springer.en_US
dc.identifier.otherhttps://doi.org/10.1186/2193-1801-3-541
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/25577
dc.language.isoenen_US
dc.publisherSpringerPlusen_US
dc.subjectCharacterizationen_US
dc.subjectCowpeaen_US
dc.subjectDiversityen_US
dc.subjectGeneticen_US
dc.subjectGenotypeen_US
dc.subjectMarkersen_US
dc.subjectSNPsen_US
dc.titleDiversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markersen_US
dc.typeArticleen_US

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