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Validation of Microsatellite Molecular Markers Linked With Resistance to Bipolaris Sorokiniana in Wheat (Triticum Aestivum L.)

Show simple item record Tembo, B. Sibiya, J. Tongoona, P. Tembo, L. 2019-07-31T12:16:45Z 2019-07-31T12:16:45Z 2017-03
dc.identifier.other DOI: 10.1017/S0021859617000144
dc.identifier.other Vol.155(7)
dc.description.abstract Spot blotch disease caused by Bipolaris sorokiniana (Sacc.) Shoem causes yield losses and reduces grain quality in wheat. Molecular markers reported to be linked with resistance to B. sorokiniana could accelerate the identification of resistant genotypes as they are independent of the environmental effect. However, before they can be utilized for marker assisted selection (MAS), validation in an independent population is required. The objective of the present study was therefore to validate three simple sequence repeat (SSR) molecular markers ( Xwgm570, Xgwm544 and Xgwm437 ) linked with resistance to B. sorokiniana. The markers were validated using 66 wheat genotypes comprising 11 parental genotypes and 55 F 2 progenies. Single marker analysis was performed using simple linear regression to ascertain the relationship between the marker and the trait. All the markers were confirmed to be associated with resistance. They all gave significant association with resistance to B. sorokiniana. The markers amplified DNA fragments in the resistant parental genotypes that were similar to those observed in resistant F 2 progenies, but absent in the susceptible ones. Hence, these markers could be useful in increasing the efficiency of selection for resistance to B. sorokiniana in wheat breeding. Since the R² values are low, a combination of two or three SSR markers can be employed during MAS. This was evident by the multiple linear equation which gave a combined R² value of 18·0%, obtained from the inclusion of all three markers. en_US
dc.language.iso en en_US
dc.publisher Validation of microsatellite molecular markers linked with resistance to Bipolaris sorokiniana in wheat (Triticum aestivum L.) en_US
dc.subject Crop Yield en_US
dc.subject DNA en_US
dc.subject Environmental Effect en_US
dc.subject Fungal Disease en_US
dc.subject Fungus en_US
dc.subject Genetic Marker en_US
dc.subject Wheat en_US
dc.subject Genotype en_US
dc.subject Selective Breeding en_US
dc.subject Bipolaris Sorokiniana en_US
dc.subject Triticum Aestivum en_US
dc.title Validation of Microsatellite Molecular Markers Linked With Resistance to Bipolaris Sorokiniana in Wheat (Triticum Aestivum L.) en_US
dc.type Article en_US

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