Serological detection of Dioscorea alata potyvirus on white yams (Dioscorea rotundata) in Ghana
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Date
2007-01
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Publisher
Journal of Plant Sciences
Abstract
A baseline survey was conducted in ten important yam growing districts in Ashanti and Brong Ahafo regions of Ghana during June/July, 2004 to detect the incidence of viral diseases of white yam (Dioscorea rotundata, Poir). One hundred and seventy six leaf samples were collected and virus-indexed using the Enzyme Linked Immunosorbent Assay (ELISA) technique. Four yam virus antisera homologous to Yam mosaic virus (YMV), genus Potyvirus, Cucumber mosaic virus (CMV), genus Cucumovirus, Dioscorea alata potyvirus (DAV), genus Potyvirus and Dioscorea alata badnavirus genus Badnavirus were used. None of the antisera raised against Dioscorea alata badnavirus (DaV) and Cucumber mosaic virus (CMV) reacted positively. Antiserum raised against YMV reacted positively to antigens in 38% of the samples, while 20.5% reacted positively to DAV antigens. Eleven percent of the total samples collected reacted to both YMV and DAV antisera. Forty one percent of the samples however reacted negatively with all the antisera. The detection of DAV on white yam is the first evidence to be reported in Ghana. This finding provides useful information for the management of viral diseases of yam in Ghana. © 2007 Academic Journals Inc.
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Keywords
Dioscorea alata potyvirus, Dioscorea rotundata, White yam, Yam mosaic potyvirus