Browsing by Author "Usui, M."
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Item Plasmodium falciparum sexual differentiation in malaria patients is associated with host factors and GDV1-dependent genes(Nature Communications, 2019-05) Usui, M.; Prajapati, S.K.; Ayanful-Torgby, R.; Acquah, F.K.; Cudjoe, E.; Kakaney, C.; Amponsah, J.A.; Obboh, E.K.; Reddy, D.K.; Barbeau, M.C.et.al.Plasmodium sexual differentiation is required for malaria transmission, yet much remains unknown about its regulation. Here, we quantify early gametocyte-committed ring (gc-ring) stage, P. falciparum parasites in 260 uncomplicated malaria patient blood samples 10 days before maturation to transmissible stage V gametocytes using a gametocyte conversion assay (GCA). Seventy six percent of the samples have gc-rings, but the ratio of gametocyte to asexual-committed rings (GCR) varies widely (0–78%). GCR correlates positively with parasitemia and is negatively influenced by fever, not hematocrit, age or leukocyte counts. Higher expression levels of GDV1-dependent genes, ap2-g, msrp1 and gexp5, as well as a gdv1 allele encoding H217 are associated with high GCR, while high plasma lysophosphatidylcholine levels are associated with low GCR in the second study year. The results provide a view of sexual differentiation in the field and suggest key regulatory roles for clinical factors and gdv1 in gametocytogenesis in vivo.Item Plasmodium falciparum sexual differentiation in malaria patients is associated with host factors and GDV1-dependent genes(Nature Communications, 2019) Usui, M.; Prajapati, S.K.; Ayanful-Torgby, R.; Acquah, F.K.; Cudjoe, E.; Kakaney, C.; Amponsah, J.A.; Obboh, E.K.; Reddy, D.K.; Barbeau, M.C.; Simons, L.M.; Czesny, B.; Raiciulescu, S.; Olsen, C.; Abuaku, B.K.; Amoah, L.E.; Williamson, K.C.Plasmodium sexual differentiation is required for malaria transmission, yet much remains unknown about its regulation. Here, we quantify early gametocyte-committed ring (gc-ring) stage, P. falciparum parasites in 260 uncomplicated malaria patient blood samples 10 days before maturation to transmissible stage V gametocytes using a gametocyte conversion assay (GCA). Seventy six percent of the samples have gc-rings, but the ratio of gametocyte to asexual-committed rings (GCR) varies widely (0–78%). GCR correlates positively with parasitemia and is negatively influenced by fever, not hematocrit, age or leukocyte counts. Higher expression levels of GDV1-dependent genes, ap2-g, msrp1 and gexp5, as well as a gdv1 allele encoding H217 are associated with high GCR, while high plasma lysophosphatidylcholine levels are associated with low GCR in the second study year. The results provide a view of sexual differentiation in the field and suggest key regulatory roles for clinical factors and gdv1 in gametocytogenesis in vivo. © 2019, The Author(s).Item Publisher Correction: Plasmodium falciparum sexual differentiation in malaria patients is associated with host factors and GDV1-dependent genes(Nature Communications, 2019) Usui, M.; Prajapati, S.K.; Ayanful-Torgby, R.; Acquah, F.K.; Cudjoe, E.; Kakaney, C.; Amponsah, J.A.; Obboh, E.K.; Reddy, D.K.; Barbeau, M.C.; Simons, L.M.; Czesny, B.; Raiciulescu, S.; Olsen, C.; Abuaku, B.K.; Amoah, L.E.; Williamson, K.C.The original version of this Article contained an error in Fig. 6, in which the right panel showing gametocytemia in the 6th cycle was flipped horizontally. This has been corrected in both the PDF and HTML versions of the Article. © 2019, The Author(s).