Browsing by Author "Suzuki, Y."
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Item Evolution of a G6P[6] rotavirus strain isolated from a child with acute gastroenteritis in Ghana, 2012(Journal of General Virology, 2015-08) Agbemabiese, C.A.; Nakagomi, T.; Suzuki, Y.; Armah, G.; Nakagomi, O.Unusual human G6P[6] rotavirus A (RVA) strains have been reported sporadically in Europe and Africa, but how they evolved was not fully understood. The whole genome of a Ghanaian G6P[6] strain designated PML1965 (2012) was analysed to understand how it evolved in Africa and to learn how its G6 VP7 gene was related to that of rotaviruses of human and artiodactyl origin. The genotype constellation of RVA/Human-wt/GHA/PML1965/2012/G6P[6] was G6-P-[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2. It shared sublineages with G6P[6] strains previously detected in Italy and Africa in all genome segments except the VP6 gene of a few Burkinabe and Cameroonian strains and both the VP6 and NSP4 genes of Guinea Bissau strains. The VP7 gene of the G6P[6] strains appeared to derive from those of human G6P[9] strains, and they were distantly related to the VP7 genes of artiodactyl G6 or human G6P[14] strains. The time of the most recent common ancestor of the VP7 sequences of G6P[6] strains was estimated to be the year 1998. The evolutionary rates of the VP7 genes in bovine and human G6 rotaviruses were 6.93 × 10(-4) and 3.42 × 10(-3) nucleotide substitutions site(-1) year(-1), respectively, suggesting an accelerated adaptive process in the new host. The sequences of the remaining 10 genome segments of PML1965 clustered with those of G2 and G8 human rotaviruses detected in Africa possessing the DS-1-like genetic background. In conclusion, PML1965 evolved from G2 or G8 RVA strains with DS-1-like background, acquiring the G6 VP7 gene from a human G6P[9] RVA and not from an artiodactyl G6 RVA strain.Item Genome-wide association study identifies seven novel susceptibility loci for primary open-angle glaucoma(Oxford University Press, 2018) Shiga, Y.; Akiyama, M.; Nishiguchi, K.M.; Sato, K.; Shimozawa, N.; Takahashi, A.; Momozawa, Y.; Hirata, M.; Matsuda, K.; Yamaji, T.; Iwasaki, M.; Tsugane, S.; Oze, I.; Mikami, H.; Naito, M.; Wakai, K.; Yoshikawa, M.; Miyake, M.; Yamashiro, K.; Kashiwagi, K.; Iwata, T.; Mabuchi, F.; Takamoto, M.; Ozaki, M.; Kawase, K.; Aihara, M.; Aihara, M.; Yamamoto, T.; Kiuchi, Y.; Nakamura, M.; Ikeda, Y.; Sonoda, K.-H.; Ishibashi, T.; Nitta, K.; Iwase, A.; Shirato, S.; Oka, Y.; Satoh, M.; Sasaki, M.; Fuse, N.; Suzuki, Y.; Cheng, C. -Y.; Khor, C.C.; Baskaran, M.; Perera, S.; Aung, T.; Vithana, E. N.; Cooke Bailey, J.N; Kang, J.H.; Pasquale, L.R.; Haines, J.L.; Wiggs, J.L.; Burdon, K.P.; Gharahkhani, P.; Hewitt, A.W.; Mackey, D.A.; MacGregor, S.; Craig, J.E.; Rand Allingham, R.; Hauser, M.; Ashaye, A.; Budenz, D.L.; Akafo, S.; Williams, S.E.I.; Kamatani, Y.; Nakazawa, T.; Kubo, M.Primary open-angle glaucoma (POAG) is the leading cause of irreversible blindness worldwide for which 15 diseaseassociated loci had been discovered. Among them, only 5 loci have been associated with POAG in Asians. We carried out a genome-wide association study and a replication study that included a total of 7378 POAG cases and 36 385 controls from a Japanese population. After combining the genome-wide association study and the two replication sets, we identified 11 POAG-associated loci, including 4 known (CDKN2B-AS1, ABCA1, SIX6 and AFAP1) and 7 novel loci (FNDC3B, ANKRD55-MAP3K1, LMX1B, LHPP, HMGA2, MEIS2 and LOXL1) at a genome-wide significance level (P<5.0×10-8), bringing the total number of POAG-susceptibility loci to 22. The 7 novel variants were subsequently evaluated in a multiethnic population comprising non-Japanese East Asians (1008 cases, 591 controls), Europeans (5008 cases, 35 472 controls) and Africans (2341 cases, 2037 controls). The candidate genes located within the new loci were related to ocular development (LMX1B, HMGA2 and MAP3K1) and glaucoma-related phenotypes (FNDC3B, LMX1B and LOXL1). Pathway analysis suggested epidermal growth factor receptor signaling might be involved in POAG pathogenesis. Genetic correlation analysis revealed the relationships between POAG and systemic diseases, including type 2 diabetes and cardiovascular diseases. These results improve our understanding of the genetic factors that affect the risk of developing POAG and provide new insight into the genetic architecture of POAG in Asians. © The Author(s) 2018. Published by Oxford University Press. All rights reserved.Item Seasonal Changes in the Plant Growth-Inhibitory Effects of Rosemary Leaves on Lettuce Seedlings(Plants, 2022) Appiah, K.S.; Omari, R.A.; Onwona-Agyeman, S.; Amoatey, C.A.; Ofosu-Anim, J.; Smaoui, A.; Arfa, A.B.; Suzuki, Y.; Oikawa, Y.; Okazaki, S.; Katsura, K.; Isoda, H.; Kawada, K.; Fujii, Y.Plant biodiversity has been studied to explore allelopathic species for the sustainable management of weeds to reduce the reliance on synthetic herbicides. Rosemary (Rosmarinus officinalis L., syn Salvia rosmarinus Spenn.), was found to have plant growth-inhibitory effects, and carnosic acid was reported as an allelochemical in the plant. In this study, the effects of seasonal variation (2011–2012) on the carnosic acid concentration and phytotoxicity of rosemary leaves from two locations in Tunisia (Fahs and Matmata) were investigated. The carnosic acid concentration in rosemary leaves was determined by HPLC, and lettuce (Lactuca sativa L.) was used as the receptor plant in the phytotoxicity bioassay. The highest carnosic acid concentration was found in rosemary samples collected in June 2011, which also had the highest inhibitory activity. Furthermore, a significant inverse correlation (r = 0.529; p < 0.01) was found between the inhibitory activity on lettuce hypocotyl and the carnosic acid concentration in rosemary leaves. Both temperature and elevation had a significant positive correlation with carnosic acid concentration, while rainfall showed a negative correlation. The results showed that the inhibitory effects of rosemary leaf samples collected in summer was highest due to their high carnosic acid concentration. The phytotoxicity of rosemary needs to be studied over time to determine if it varies by season under field conditions.