Browsing by Author "Sugri, I."
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Item Citrus Extract Found Potent in the Control of Seed-Borne Fungal Pathogens of Pearl Millet—A Recommendation for Farmers’ Seed Saving Systems(Horticulturae, 2023) Anafo, M.A.; Sugri, I.; Asungre, P.A.; Ankamah-Yeboah, T.; Eleblu, J.S.Y.; Danquah, E.Y.Seed saving is crucial to ensure seed and food security, especially in developing countries. In Ghana, about 90% of pearl millet farmers utilise farmer-saved seed for production. Such seeds usually have a low germination rate, and may carry important seed-borne pathogens. In this study, the quality of farmer-saved seeds and the potential of botanicals to control seed-borne fungi were examined. A structured questionnaire was administered to 120 farmers from Garu, Bongo and Kassana-Nankana districts in Ghana. During the survey, 60 samples of farmer-saved seed were obtained for seed quality evaluation. Aqueous extracts of neem (Azadirachta indica) and citrus (Citrus sinensis) were prepared as seed treatments compared with a control. Fourteen (14) fungal species were associated with the seed samples, but the four dominant ones were Curvularia spp. (62%), Talaromyces spp. (53%), Aspergillus spp. (52%) and Exserohilum spp. (42%). The application of citrus extract inhibited fungal incidence (15–31.7%) compared to neem extract (33.3–93.3%) and the control (96.7–98.3%). The application of citrus extract resulted in a higher (74.7–82.8%) field germination rate compared to neem extract (62.3–73.2%) and the control (65.5–69.2%). The citrus extract was the most efficient treatment in reducing the incidence of fungi and enhancing the seed germination and vigour of pearl millet.Item A novel aphid resistance locus in cowpea identified by combining SSR and SNP markers(Plant Breeding, 2018-04) Kusi, F.; Padi, F.K.; Obeng-Ofori, D.; Asante, S.K.; Agyare, R.Y.; Sugri, I.; Timko, M.P.; Koebner, R.; Huynh, B.L.; Santos, J.R.P.; Close, T.J.; Roberts, P.A.The utility of combining simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) marker genotyping was determined for genetically mapping a novel aphid (Aphis craccivora) resistance locus in cowpea breeding line SARC 1‐57‐2 and for introgressing the resistance into elite cultivars by marker‐assisted backcrossing (MABC). The locus was tagged with codominant SSR marker CP 171F/172R with a recombination fraction of 5.91% in an F2 population from ‘Apagbaala’ x SARC 1‐57‐2. A SNP‐genotyped biparental recombinant inbred line population was genotyped for CP 171F/172R, which was mapped to position 11.5 cM on linkage group (LG) 10 (physical position 30.514 Mb on chromosome Vu10). Using CP 171F/172R for foreground selection and a KASP‐SNP‐based marker panel for background selection in MABC, the resistance from SARC 1‐57‐2 was introduced into elite susceptible cultivar ‘Zaayura’. Five BC4F3 lines of improved ‘Zaayura’ that were isogenic except for the resistance locus region had phenotypes similar to SARC 1‐57‐2. This study identified a novel aphid resistance locus and demonstrated the effectiveness of integrating SSR and SNP markers for trait mapping and marker‐assisted breeding.Item Preliminary assessment of shea butter waxing on the keeping and sensory qualities of four plantain (Musa aab) varieties(African Journal of Agricultural Research, 2010-01) Sugri, I.; Norman, J.C.; Egyir, I.; Johnson, P.N.T.Over the last few years, the production of plantain has been increasing due to the introduction of some high yielding varieties. Unfortunately, very high post-harvest losses are incurred annually due to lack of appropriate storage technologies. This study evaluated the effect of shea butter as a food-grade wax on the pre-climacteric life and sensory qualities of four plantain varieties (Apem, Apentu, Asamienu and Oniaba). Waxing was achieved by brushing shea butter on fruits surfaces. The fruit diameter before waxing (d1) and after waxing (d2) was measured and the difference (d2-d1) taken as the waxing thickness. Parameters assessed were the uniformity of ripening, gloss quality, incidence of off-flavours and disorders. Waxing thickness of 0.5 and 1 mm resulted in irregular ripening, green-soft disorder and off-flavours. However, thin layer waxing (<0.05mm) prolonged the pre-climacteric life to 22, 20, 17, and 15 days in Apem, Apentu, Oniaba and Asamienu respectively. A prolonged post-climacteric life from 4 to 6 days across varieties as well as firm texture at senescence was recorded. Shea butter waxing resulted in significant decrease (P<0.001) in physiological weight loss of 9.46% by 22 days after storage compared to control which had 21.25% by 10 days of storage. Principal component analysis of sensory attributes showed that mouth-feel, colour, flavour and tastes accounted for 36.67 % of data variation whist texture alone accounted for 21.1% of data variability. Due to increasing lobby against the use of post-harvest chemicals, as consumers now want products with high nutritional value rather than having just superior external qualities, the use of shea butter as a food-grade wax can be further explored since no safety or residual effect is foreseeable. © 2010 Academic Journals.