Browsing by Author "Quartey, E.K."
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Item Field evaluation of tomato varieties/breeding lines against tomato yellow leaf curl virus disease (TYLCV)(Pertanika Journal of Tropical Agricultural Science, 2018-02) Segbefia, M.M.; Amoatey, H.M.; Ahiakpa, J.K.; Quartey, E.K.; Appiah, A.S.; Nunoo, J.; Kusi-Adjei, R.Tomato Yellow Leaf Curl Virus (TYLCV) is currently the most devastating virus of cultivated tomatoes in tropical and subtropical regions, accounting for significant yield losses in cultivated tomato in Ghana. Severe population outbreaks of the whitefly vector (Bemisia tabaci), are usually associated with high incidence of the disease. Resistance breeding is the surest solution to TYLCV in developing viable seeds for increased tomato production in Ghana. The Wild tomato (Solanum pimpinellifolium L.) is a recognised crop Wild species (CWS) with resistance genes to different diseases including the TYLCV disease and possesses good fruit quality traits in Ghana. Three (3) cultivated tomato varieties and seven breeding lines developed from crosses between the Wild tomato and three hybrids, three backcrossed lines and the Wild tomato were evaluated with their parents against TYLCV disease under local field conditions. Field appraisal of whitefly populations, disease incidence and severity, agronomic and yield characteristics of the tomato varieties/breeding lines were undertaken to hasten selection of tolerant/resistant varieties or breeding lines in the breeding programme. Wild tomato (ISSAP = 0.31 and ISSDP = 0.76) and Woso (ISSAP = 1.90 and ISSDP = 2.27) recorded the least and highest average symptom severity on all plants (ISSAP) and diseased plants only (ISSDP); while the least and highest disease incidence was recorded by the Wild tomato (11.10%) and Roma (43.05%). Roma which recorded the highest population of whiteflies in the dry season also exhibited the highest symptom severity on all plants as well as diseased plants during the study period. There was a significant symptom relapse in Wild tomato and Woso x Wild in 6-8 WAT for both ISSAP and ISSDP. Number of fruits per plant, ISSAP and ISSDP were positively and/or inversely correlated (r = 0.98, 0.93, -0.83) with average whitefly count, percent disease incidence and yield (t/ha).Item Induced mutations for improved lycopene, total antioxidant properties and other quality factors in wild tomato (Solanum pimpinellifoliumL)(Advance Journal of Food Science and Technology, 2012) Quartey, E.K.; Amoatey, H.K.; Achel, D.G.; Klu, G.Y.P.; Mba, R.A.The objective of the study was to determine biochemical changes in (5) gamma radiation induced variant lines of wild tomato after mutation induction. Five gamma radiation-induced variant lines of wild tomato (Solanum pimpinellifolium L.), selected in the M3 generation following mutagenic treatment of seeds using three doses (150, 300 and 450 Gy, respectively) of gamma radiation from a Co-60 gamma source, were analysed for their lycopene content, total antioxidant properties, total and soluble solids as well as pH. Fruits of variant line BV-21 (deep red fruits) recorded higher lycopene contents of 146.1 mg/kg on fresh weight basis and 156.7 mg/kg on dry weight basis compared to 136 and 152 mg/kg, respectively for the control. They also recorded higher total antioxidant properties compared to the control. Fruits of variant line BV-40 (yellow fruits) recorded higher total solids of 17.9% and the lowest pH value of 4.17 compared to 12.8% and 4.36 for fruits from unirradiated plants. The highest amounts of total soluble solids (7%) were contained in fruits harvested from variant lines BV-27 (light red fruits) and BV-23 (deep red fruits) as against 5.6% for fruits from control plants. The study indicates that wild tomato has immense nutritional properties which can be further improved through mutation breeding. © Maxwell Scientific Organization, 2012.Item Intra-specific variation in West African and Asian germplasm of okra (Abelmoschus spp L.)(Science Direct (Annals of Agricultural Science), 2017-09-28) Ahiakpa, J.K.; Magdy, M.; Werner, O.; Amoatey, H.M.; Yeboah, M.A.; Appiah, A.S.; Quartey, E.K.; Ros, R.M.Ten quantitative agromorpho-economic traits, six inter-simple sequence repeat (ISSR) primers, and three sequenced regions were employed to study intra-specific genetic diversity among twenty-eight accessions of West African and Asian okra (Abelmoschus spp L.) collected from eight geographical regions of Ghana. Pod yield per plant was analysed as dependent variable in relation to other agromorphoeconomic traits, showing the correlation and contribution of each trait to crop yield. 50% germination and flowering were the most significant traits followed by plant height and average seeds per plant. Principal coordinate analysis defined three sets of traits, while Agglomerative Hierarchical Clustering (AHC) defined three clusters of the germplasms. ISSR detected very low level of polymorphism among the accessions. Testing the correlation between molecular data and morphological traits using Mantel test showed a significant positive correlation (r-value = 0.71, 0.90) with 50% flowering, fruiting and number of leaves per plant. Eclectic variation between Indiana and the rest of the accessions for both agromorpho-economic traits and molecular markers affirms its potential usefulness as a source of diverse genes for future breeding programmes. Sequencing of regions from all accessions, suggests that they are identical with a common ancestry. Outcomes of this study is timely for an ongoing okra hybridisation programme in Ghana.