Browsing by Author "Egbadzor, K.F."
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Item Diversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markers(2014-09-20) Egbadzor, K.F.; Ofori, K.; Yeboah, M.; Aboagye, L.M.; Opoku-Agyeman, M.O.; Danquah, E.Y.; Offei, S.K.Abstract Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection.Item Diversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markers(SpringerPlus, 2014-09) Egbadzor, K.F.; Ofori, K.; Yeboah, M.; Aboagye, L.M.; Opoku-Agyeman, M.O.; Danquah, E.Y.; Offei, S.K.Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection. © 2014, Egbadzor et al.; licensee Springer.Item Factors influencing rice production in the south-eastern belt of Ghana(Heliyon, 2022) Bissah, M.N.; Kotey, D.A.; Tongoona, P.; Egbadzor, K.F.; Gracen, V.; Danquah, E.Y.Ghana has great potential to produce rice for local consumption, however, the average rice produced barely meets half of the country's consumption needs. Focus group discussions (FGDs) and surveys were held within the coastal lowland rice production belt in Ghana. The FGDs were held at Okyereko and Afife followed by surveys in the two communities in addition to Dawenya and Ashaiman. The objectives were to assess the production challenges faced by farmers in the four communities and determine the rice traits preferred by the farmers. Twenty farmers were involved in each of the FGDs while 227 respondents were selected through convenient sampling for the interviews. Sixty-nine percent of respondent farmers were male, 53% were above 50 years while 44% had varied levels of education. Farmers preferences mainly related to marketable traits such as aroma (87%), taste (83%) and yield potential (78%). There was special preference for Jasmine 85 (62.8%) and Togo Marshal l (25%), an indication of the high adoption of aromatic rice varieties in the study areas. Challenges such as securing credit, input supplies, soil salinity and ageing work force were matters of grave concern to respondent farmers. Given the importance of rice cultivation in the study areas to Ghana’s quest to achieve self-sufficiency in rice, targeted and coordinated support from relevant governmental and non-governmental organizations is needed to sustain a higher level of production in the study areas.Item Farmer participation in selection within segregating populations of cowpea in Volta Region, Ghana(2015-11-01) Egbadzor, K.F.; Offei, S.K.; Danquah, E.Y.; Kotey, D.A.; Gamedoagbao, D.K.; Dadoza, M.; Yeboah, M.; Ofori, K.Abstract Background Participatory plant breeding leads to early adoption of a newly released variety as farmers would be aware of whatever advantage it might have over prior existing ones as early as possible. Cowpea farmers around Ohawu in the Volta Region of Ghana where the crop is one of the most important cash crops had no variety specifically developed for their environment. This research followed farmers’ interest identified earlier for the development of varieties with preferred consumer traits. Methods Farmers were chosen from 25 communities within the cowpea growing area by the help of agricultural extension officers. Fifty farmers including 25 males and females each, 7 scientists and 15 other persons with different backgrounds were involved in the selection exercise. The final plants selected were based on the total number of participants’ endorsement and analysis of variance for their seed size. Results Selections were made from six F3 populations with parents of various unique traits obtained from Botswana, Ghana, Nigeria, Senegal, or United States of America. Four most liked cowpea plants from each of the six populations were selected to be advanced and tried in multi-locations towards probable release. Conclusions Participants were very enthused with the selection exercise hoping of seeing some of their selections resulting into varieties. Farmers were interested in selecting plants with long multiple pods and early maturing. The exercise was educative for all participants.Item Farmer participation in selection within segregating populations of cowpea in Volta Region, Ghana(Agriculture & Food Security, 2015-11) Egbadzor, K.F.; Offei, S.K.; Danquah, E.Y.; Kotey, D.A.; Gamedoagbao, D.K.; Dadoza, M.; Yeboah, M.; Ofori, K.Background Participatory plant breeding leads to early adoption of a newly released variety as farmers would be aware of whatever advantage it might have over prior existing ones as early as possible. Cowpea farmers around Ohawu in the Volta Region of Ghana where the crop is one of the most important cash crops had no variety specifically developed for their environment. This research followed farmers’ interest identified earlier for the development of varieties with preferred consumer traits. Methods Farmers were chosen from 25 communities within the cowpea growing area by the help of agricultural extension officers. Fifty farmers including 25 males and females each, 7 scientists and 15 other persons with different backgrounds were involved in the selection exercise. The final plants selected were based on the total number of participants’ endorsement and analysis of variance for their seed size. Results Selections were made from six F3 populations with parents of various unique traits obtained from Botswana, Ghana, Nigeria, Senegal, or United States of America. Four most liked cowpea plants from each of the six populations were selected to be advanced and tried in multi-locations towards probable release. Conclusions Participants were very enthused with the selection exercise hoping of seeing some of their selections resulting into varieties. Farmers were interested in selecting plants with long multiple pods and early maturing. The exercise was educative for all participants.Item Genetic Control and Breeding for Seed Size and Colour in Cowpea [Vigna Unguiculata (L.) Walp](University of Ghana, 2014-12) Egbadzor, K.F.; University of Ghana, College of Basic and Applied Sciences, West Africa Centre for Crop ImprovementConsumer preference is very important in acceptance of an improved crop variety. Participatory rural appraisal (PRA) was therefore conducted on preferred traits of cowpea [Vigna unguiculata (L) Walp] among Ghanaian consumers in Ketu North, Ho and Akatsi districts. It was confirmed that the age long preference for large white cowpea among Ghanaian consumers still exists. Based on the results of the PRA, cowpea germplasm was assembled and used in genetic studies on seed coat colour and size. The total number of germplasm used for the various experiments was 126 from which 111 were from Ghana. Sixteen morphological traits were used in characterisation which clustered the improved varieties together indicating selection for similar traits. Molecular characterisation with 458 Single Nucleotide Polymorphic (SNP) markers clustered the germplasm based on seed coat colour and showed close relationship between improved varieties and materials from similar breeding programmes when analyzed with Darwin software. The software package structure was used to separate accessions into three groups, and the program correctly identified varieties that are known to have hybrid origins such as Zaayura and CB27. The SNP markers were also used in association mapping for cowpea seed size with Genstat statistical package. Eighteen of the SNP markers were observed to be highly associated with seed size and could be used in marker assisted selection for the trait. Eight of these 18 SNPs, one each located on Linkage Groups (LGs) 1, 5, 7, 10 and 11 and three on LG 4 were associated with both seed weight and thickness. Generation mean analysis (GMA) for seed size was conducted using CB27 and Gh3710 as the large and small seeded parents respectively. Results of the GMA analyzed with Genstat and SAS indicated that both fixable and non-fixable gene actions were important in seed size inheritance. Eight genes were estimated to control seed size of cowpea from the experiment. The broad and narrow sense heritability estimates were 84% and 35% respectively. Duplicate gene action was observed to control seed size inheritance in cowpea. Small seed size was partially dominant over large seed size. Seven genotypes were used in hybridization and the segregating populations used in seed coat colour inheritance study in cowpea. It was realized that segregation for seed coat colour could be complex resembling quantitative inheritance depending on the parental genotypes. It was therefore suggested that the use of quantitative and molecular approaches could help in better understanding of seed coat colour inheritance in cowpea. Participatory selection with farmers was done from six different F3 populations of cowpea from which four plants each were selected for further evaluation toward possible varietal development.