Browsing by Author "Anani, P.A."
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Item Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential(Cogent Engineering, 2019-04-30) Asimeng, B.O.; Tiburu, E.K.; Kaufmann, E.E.; Paemka, L.; Hayford, C.F.; Essien-Baidoo, S.; Dzikunu, O.K.; Anani, P.A.This study reports the electrochemical activities of a novel ion substituted-Hydroxyapatite (HAp) material in contact with HeLa cells. The work was performed to evaluate the inhibitory effects of various concentrations of HAp on ion transfer mechanisms in HeLa cells. The materials (n = 2: HAp1 and HAp3) were prepared at different stirring times from Achatina achatina snail shells and phosphate-containing solution. The structure of the materials and the trace elements concentration were evaluated using x-ray diffractometry and infrared spectrometry as well as atomic absorption spectroscopy. Electrochemical studies conducted on the cells after 30 min of exposure to the materials demonstrated different responses as elucidated by cyclic voltammetry. The voltammograms revealed HAp1 to be non-redox whereas HAp3 was redox active. Minimal concentrations of HAp1 showed high anodic peak current when compared to the HeLa cells alone, indicating a hyperpolarization of the cells. The peak current gradually reduced as the concentration of HAp1 was increased, and then followed by a sudden rise suggesting inhibition of the cell action potential. HAp3 showed a wavy pattern of the anodic peak current when the material concentration was varied. Peak currents of 0.92±0.03 nA and 0.57±0.01 nA were recorded for HAp1 and HAp3, respectively at the highest concentration of 5 µL. The results suggest that different inhibitory mechanisms are at play on the voltage-gated ion channels of the cells, indicating the possibility of using the materials to achieve different cancer proliferation inhibition.Item Preparation and characterization of hydroxyapatite from Achatina achatina snail shells: effect of carbonate substitution and trace elements on defluoridation of water(Journal of Asian Ceramic Societies, 2018-07) Asimeng, B.O.; Fianko, J.R.; Kaufmann, E.E.; Tiburu, E.K.; Hayford, C.F.; Anani, P.A.; Dzikunu, O.K.A novel hydroxyapatite [HAp: Ca10 (PO4)6(OH)2] material for defluoridation was prepared from Achatina achatina (AA) snail shells using a modified chemical precipitation method. X-ray diffractometry and atomic absorption spectrometry revealed carbonate substitution as a function of stirring conditions. Stirring time was varied to control crystallite size and trace element concentrations. In addition, Infrared spectra, cyclic voltammograms, and ion exchange profiles confirmed the functional groups, the surface mass concentration and the fluoride removal efficiency, respectively. It was observed that the samples prepared after 1 hr optimal stirring times reduced fluoride concentration from to without affecting the overall pH conditions of the water, whereas beyond this time frame, low uptake of the fluoride ions was obtained with increasing pH conditions. It was also observed that crystallite size did not affect the removal capacity of the samples. The results demonstrated herein the possibility of using locally prepared AA shells for water purification and other environmental remediation applications.