Abstract:
The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human
G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were
constructed that supported recovery of recombinant RIX4414-like viruses by reverse
genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing
improvements to its efficacy.