Abstract:
The cytotoxicity of five compounds previously isolated from the gorgonian Acalycigorgia sp was evaluated against human myeloid leukemia cells HL-60 and T-lymphocyte Jurkat cells. The tetrazolium-based colorimetric (MTT) assay was used to assess cytotoxicity of the compounds. They were found to be cytotoxic against the cell lines tested, and their IC50 values were comparable to that of curcumin which had IC50 values of 9.0μM and 6.8μM in HL-60 and Jurkat cells, respectively. Compound 1 was the most cytotoxic with IC50 values of 15.0μM and 21.9μM in HL-60 and Jurkat cells, respectively. The least cytotoxic was compound 5 which had IC50 values of 92.0μM and 123.8μM in HL-60 and Jurkat cells, respectively. The lower cytotoxicity of compound 5 could be attributed to the bulky fatty acid moiety attached at C6 of the main eunicellin skeleton. The GST activity of the five compounds was also studied and the results showed that the compounds had only moderate GST inhibitory activities. Compound 3 showed the strongest GST inhibitory activity with IC50 value of 0.67mM compared to the IC50 value of 0.01mM given by ETA which was used as a positive control. In addition, the five compounds tested showed very mild radical scavenging activity in the DPPH test. © Research India Publications.
