Issah, I.Arko-Mensah, J.Rozek, L.S.Rentschler, K.Agyekum, T.P.Dwumoh, D.Batterman, S.Robins, T.G.Fobil, J.N.2022-01-062022-01-062021https://doi.org/10.1080/09603123.2021.1969007http://ugspace.ug.edu.gh/handle/123456789/37499Research ArticleThis study examined the associations between PM (2.5 and 10) and global DNA methylation among 100 e-waste workers and 51 non-e-waste work ers serving as controls. Long interspersed nucleotide repetitive elements 1 (LINE-1) was measured by pyrosequencing. Personal PM2.5 and PM10 were measured over a 4-hour work-shift using real-time particulate moni tors incorporated into a backpack . Linear regression models were used to assess the association between PM and LINE-1 DNA methylation. The concentrations of PM2.5 and PM10 were significantly higher among the e-waste workers than the controls (77.32 vs 34.88, p < 0.001 and 210.21 vs 121.92, p < 0.001, respectively). PM2.5 exposure was associated with increased LINE-1 CpG2 DNA methylation (β = 0.003; 95% CI; 0.001, 0.006; p = 0.022) but not with the average of all 4 CpG sites of LINE-1. In summary, high levels of PM2.5 exposure was associated with increased levels of global DNA methylation in a site-specific manner.enDNA methylationLINE-1PM2.5PM10e-wastee-waste workersArticle