Huang, S.Elsayed, S.S.Lv, M.Tabudravu, J.Rateb, E.M.Gyampoh, R.Kyeremeh, K.Ebel, R.Jaspars, M.Deng, Z.Yu, YI.Deng, H.2018-08-312018-08-312015-12Huang et al., 2015,Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps Chemistry & Biology 22, 1633–1642http://dx.doi.org/10.1016/j.chembiol.2015.10.012http://ugspace.ug.edu.gh/handle/123456789/23905Neocarazostatin A (NZS) is a bacterial alkaloid with promising bioactivities against free radicals, featuring a tricyclic carbazole nucleus with a prenyl moiety at C-6 of the carbazole ring. Here, we report the discovery and characterization of the biosynthetic pathway of NZS through genome mining and gene inactivation. The in vitro assays characterized two enzymes: NzsA is a P450 hydroxylase and NzsG is a new phytoene-synthase-like prenyltransferase (PTase). This is the first reported native PTase that specifically acts on the carbazole nucleus. Finally, our in vitro reconstituted experiment demonstrated a coupled reaction catalyzed by NzsG and NzsA tailoring the NZS biosynthesis.enNeocarazostatin A (NZS)bacterial alkaloidtricyclic carbazole nucleuscarbazole ringprenyl moiety at C-6BiosynthesisHydroxylationSequentialCarbazole PrenylationArticle