Dzudzor, B.Huynh, L.Thai, M.Bliss, J.M.Nagaoka, Y.Wang, Y.Ch'ng, T.H.Jiang, M.Martin, K.C.Colicelli, J.2012-05-182017-10-192012-05-182017-10-192010Mol Cell Neurosci. 43(1): 108-16http://197.255.68.203/handle/123456789/1554The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snai1 was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons.enArticle