Department of Nutrition and Dietetics

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    n-3 long-chain PUFA promote antibacterial and inflammation-resolving effects in Mycobacterium tuberculosis-infected C3HeB/FeJ mice, dependent on fatty acid status
    (Cambridge University Press, 2022) Nienaber, A.; Ozturk, M.; Dolman, R.; Blaauw, R.; Zandberg, L.L.; Rensburg, S.v; Britz, M.; Hayford, F.E.A.; Brombacher, F.; Loots, D.T.; Smuts, C.M.; Parihar, S.P.; Malan, L.
    Non-resolving inflammation is characteristic of tuberculosis (TB). Given their inflammation-resolving properties, n-3 long-chain PUFA (n-3 LCPUFA) may support TB treatment. This research aimed to investigate the effects of n-3 LCPUFA on clinical and inflammatory outcomes of Mycobacterium tuberculosis-infected C3HeB/FeJ mice with either normal or low n-3 PUFA status before infection. Using a two-by-two design, uninfected mice were conditioned on either an n-3 PUFA-sufficient (n-3FAS) or -deficient (n-3FAD) diet for 6 weeks. One week post-infection, mice were randomised to either n-3 LCPUFA supplemented (n-3FAS/n-3þ and n-3FAD/n-3þ) or continued on n-3FAS or n-3FAD diets for 3 weeks. Mice were euthanised and fatty acid status, lung bacterial load and pathology, cytokine, lipid mediator and immune cell phenotype analysed. n-3 LCPUFA supplementation in n-3FAS mice lowered lung bacterial loads (P = 0·003), T cells (P = 0·019), CD4þ T cells (P = 0·014) and interferon (IFN)-γ (P < 0·001) and promoted a pro-resolving lung lipid mediator profile. Compared with n-3FAS mice, the n-3FAD group had lower bacterial loads (P = 0·037), significantly higher immune cell recruitment and a more pro-inflammatory lipid mediator profile, however, significantly lower lung IFN-γ, IL-1α, IL-1β and IL-17, and supplementation in the n-3FAD group provided no beneficial effect on lung bacterial load or inflammation. Our study provides the first evidence that n-3 LCPUFA supplementation has antibacterial and inflammation-resolving benefits in TB when provided 1 week after infection in the context of a sufficient n-3 PUFA status, whilst a low n-3 PUFA status may promote better bacterial control and lower lung inflammation not benefiting from n-3 LCPUFA supplementation.
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    Predictors of undernutrition and anemia among children aged 6–24 months in a low-resourced setting of Ghana: a baseline survey
    (Emerald Publishing Ltd, 2021) Appiah, C.A.; Mensah, F.O.; Hayford, F.E.A.; Awuuh, V.A.; Kpewou, D.E.
    Purpose – The purpose of this study was to identify the predictors of child undernutrition and anemia among children 6–24 months old in the East Mamprusi district, Northern region, Ghana. Design/methodology/approach – This cross-sectional study recruited 153 children and their mothers. Weight, height and hemoglobin levels of the children were measured. A structured questionnaire based on the World Health Organization’s indicators for assessing infant and young child feeding practices was used to collect data on parents’ socioeconomic status, household characteristics, hygiene and sanitation practices, mothers’ knowledge on feeding practices such as child’s meal frequency and dietary diversity and child morbidity within the past two weeks. Predictors of child nutritional status were determined using multinomial logistic regression analysis. Findings – Underweight in the children was significantly predicted by maternal knowledge on protein foods (AOR 5 0.045, p 5 0.008), time of initiation of complementary feeding (AOR 5 0.222, p 5 0.032) and maternal age (AOR 5 9.455, p 5 0.017). Feeding child from separate bowls (AOR 5 0.239, p 5 0.005), minimum meal frequency per child’s age (AOR 5 0.189, p 5 0.007) and time of initiation of complementary feeding (AOR 5 0.144, p 5 0.009) were significant determinants of stunting among the children. Exclusive breast feeding (AOR 5 7.975, p 5 0.012) and child’s past morbidity (AOR 5 0.014, p 5 0.001) significantly contributed to anemia among the children. Research limitations/implications – This is a cross-sectional study and cannot establish causality. The small sample size also limits the generalizability of study findings. However, findings of the study highlight factors which could potentially influence the high rate of child undernutrition in the study setting. Practical implications – This study identifies determinants of undernutrition in the East Mamprusi district, an underresourced area in Ghana. This information could inform the development/reformulation of locally sensitive key messages and targeted intervention strategies to curb the high levels of child undernutrition in the East Mamprusi district of Ghana. Originality/value – This study identifies maternal care practices as key potential drivers of undernutrition in a low-resource setting known for high prevalence of child undernutrition. It suggests insight for large-scale studies on the predictors of child undernutrition in Northern Ghana and other resource-poor settings.
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    Beneficial effect of long-chain n-3 polyunsaturated fatty acid supplementation on tuberculosis in mice
    (Elsevier, 2021) Nienaber, A.; Oztur, M.; Dolman, R.C.; Zandberg, L.; Hayford, F.E.A.; Brombacher, F.; Blaauw, R.; Smuts, C.M.; Pariha, S.P.; Malan, L.
    Intakes of the omega-3 essential fatty acids (n-3 EFAs) are low in the general adult population, with high n-6/n-3 polyunsaturated fatty acid (PUFA) ratios and the accompanying suboptimal n-3 PUFA status. Eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) have antibacterial and inflammation-resolving effects in tuberculosis (TB). However, whether switching to a diet with optimum n-3 EFA intake after the infection has comparable benefits has not been investigated. We aimed to compare the effects of a diet with sufficient n-3 EFA content in an acceptable n-6/n-3 PUFA ratio for rodents ((n-3)eFAS group) with those on the same diet supplemented with EPA and DHA (EPA/DHA group) in Mycobacterium tuberculosis (Mtb)-infected C3HeB/FeJ mice with a low n-3 PUFA status. Mice were conditioned on an n-3 PUFA-deficient diet with a high n-6/n-3 PUFA ratio for 6 weeks before Mtb infection and randomized to either (n-3)eFAS or EPA/DHA diets 1 week post-infection for 3 weeks. At endpoint, EPA and DHA compositions were higher and arachidonic acid, osbond acid, and total n-6 LCPUFAs lower in all lipid pools measured in the EPA/DHA group (all P < 0.001). Percentage body weight gain was higher (P = 0.017) and lung bacterial load lower (P < 0.001) in the EPA/DHA group. Additionally, the EPA/DHA group had a more pro-resolving lung lipid mediator profile and lower lung in IL-1α and IL-1β concentrations (P = 0.023, P = 0.049). Inverse correlations were found between the lung and peripheral blood mononuclear cell EPA and DHA and selected pro-inflammatory cytokines. These are the first findings that indicate that EPA/DHA supplementation provides benefits superior to a diet with sufficient n-3 EFAs concerning bacterial killing, weight gain and lung inflammation resolution in Mtb-infected mice with a low n-3 PUFA status. Therefore, EPA and DHA may be worth considering as adjunct TB treatment.
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    Proximate composition and serving sizes of selected composite Ghanaian soups
    (African Journal of Food, Agriculture, Nutrition and Development, 2020-05) Hayford, F.E.A.; Appiah, C.A.; Samwini, A.M.; Brown, P.K.; Asamoah-Boakye, O.
    Soups are major components of Ghanaian dishes. The composite nature of Ghanaian soups, in general, tend to make the calorie and macronutrient composition highly variable. This study sought to determine the proximate composition of selected Ghanaian soups from defined recipes and to photographically present quantities of the soups, measured with common Ghanaian household food measures, equivalent to servings of protein and carbohydrates. Two types of each conveniently selected soup (light soup, groundnut soup, kontomire (cocoyam leaves) soup and palm nut soup) were prepared; one with animal protein and the other without. The soups prepared include; LS1- light soup with garden eggplant (African eggplant); LS2- light soup with garden eggplant and chicken GS1- groundnut (peanut butter) soup without animal protein; GS2- groundnut soup with chicken; PS1- palm nut soup without animal protein; PS2- palm nut soup with smoked catfish and herrings. Proximate analysis was done to determine macronutrient content of all the soups. Each soup was measured using a household food measure (150 ml soup ladle) to obtain a 15 g carbohydrate and 7 g protein equivalent and then photographed. Carbohydrate content of the soups ranged between 1.18 g-8.43 g, protein was between 0.34 g-4.31 g and 0.14 g-7.78 g for fat per 100 g of edible portion. Palm nut soup with animal source protein had the highest carbohydrate (8.43 ± 0.86 g) and fibre (1.99 ±0.22 g) content. Soups with the highest protein and fat content were GS2 (4.29 ± 0.14 g) and PS1 (20.39 ± 3.54 g) respectively. Serving sizes of the soups prepared ranged approximately between 1-8½ soup ladles (using 150 ml soup ladle) per 15 g carbohydrate equivalence and 1-13 soup ladles (using 150 ml soup ladle) per 7 g protein equivalence. It took variable quantities of the prepared soups to obtain the target serving sizes with PS2 having the least quantity (~1 soup ladle) for 15 g carbohydrate serving and PS1 having the least amount (~1 soup ladle) for 7 g protein serving. The large serving size of most of the soups required to meet a serving of protein implied that those soups should be eaten together with food sources rich in protein, in order to appreciably enhance the protein intake of individuals.
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    A pilot study comparing bioelectrical impedance analysis and body mass index in determining obesity among staff of a Ghanaian University
    (Nutrition and Food Science, 2018-10) Hayford, F.E.A.; Appiah, C.A.; Al Hassan, T.; Asamoah-Boakye, O.; Asante, M.
    Purpose In Ghana, the body mass index (BMI) is widely used in clinical practice in assessing weight status, but it is limited as a measure of adiposity. The purpose of this study was to compare bioelectrical impedance analysis (BIA) and body mass index (BMI) methods in determining obesity among some Ghanaians. Design/methodology/approach This was a cross-sectional survey involving 134 participants whose BMI were determined. Percentage body fat mass (%BF) and percentage visceral fat (%VF) were obtained by BIA using a hand-to-hand Omron body composition monitor with a weighing scale. Findings Based on the WHO BMI criteria, 6.0 per cent of the participants were obese. However, according to BIA 18.7 and 20.9 per cent of the participants were obese according to % BF and %VF, respectively. The BMI and %BF showed higher prevalence of obesity among female participants (8.2 and 34.4 per cent, respectively) than male participants (4.1 and 5.5 per cent, respectively), whereas for %VF, obesity was higher among male participants than female participants (26.0 per cent, 14.8 per cent). There was significant positive correlation between BMI and % BF (r = 0.604, p = 0.001); and between BMI and %VF (r = 0.555, p = 0.001). Research limitations/implications There are discrepancies in the prevalence of obesity in the study population as measured by BMI and BIA methods. This suggests that the BMI and BIA may not be reliable tools for assessing obesity in this population. Further studies are needed to determine the cut-offs for BMI and BIA that are associated with metabolic risk in the population. The small sample size limits the generalizability of findings of this study. Originality/value Body composition tends to vary by ethnicity and race; hence, it is essential to determine the appropriate tool for assessing adiposity in African populations for prompt and targeted interventions.