Browsing by Author "Adu, F.D."

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Plaque and growth characteristics of different polioviruses isolated from acute flaccid paralysis in Northern Nigeria
(East African Medical Journal 85(3):137-41, 2008) Sule, W.F.; Oyedele, O.I.; Osei-Kwasi, M.; Odoom, J.K.; Adu, F.D.
Objective: To determine some virulent trait-related properties of poliovirus isolates from children with acute flaccid paralysis following vaccination with oral polio vaccine (OPV). Design: Six polioviruses earlier characterised into wild, vaccine-derived and OPV-like were studied using the plaque morphology and growth kinetics at supra-optimal temperature. Setting: Department of Virology, University of Ibadan, Nigeria. Subjects: Polio isolates from six children who developed acute flaccid paralysis following vaccinations with various doses of OPV were used. All the children were located in the Northern part of the country where poliovirus is still circulating. Main Outcome Measures: The two vaccine-derived polioviruses acquired wild type characteristics. Results: All the six poliovirus isolates developed different forms of plaques ranging from tiny, small and large. The plaque formed could however not be used to identify the different isolates. Growth of the different isolates at supra-optimal temperature showed that the three wild polioviruses grew to a higher titre when compared with the Sabin 2 control. The two vaccine derived isolates behaved like the wild poliovirus while the OPV-like virus acquired an intermediate characteristics between wild and sabin. Conclusion: The wild polioviruses represented in this study are among the last vestiges of the circulating polioviruses found in the world. It is possible that the observed biological properties of wild types 1 and 3 described in the study are typical of the West African polioviruses. These properties will provide useful previews to the final identification of some important clinical isolates especially type 1 which may grow rapidly in cell culture.
Some genetic characteristics of sabin-like poliovirus isolated from acute flaccid paralysis cases in Nigeria
(African Journal of Biotechnology, 2003-11) Adu, F.D.; Iber, J.; Harry, T.; Burns, C.; Oyedele, O.; Adeniji, J.A.; Ossei-Kwasi, M.; Kilpatrick, D.; Tomori, O.; Kew, O.
A total of 34 sabin strains of the poliovirus isolated from 22 children with 60-day follow-up residual acute flaccid paralysis (AFP) were genetically characterized and screened for any form of recombination. Sequence analysis of the 906-nucleotide capsid showed that all the isolates were similar to their original sabin serotypes, however two of the viruses had drifted in their 3D noncapsid regions toward a sabin-sabin and sabin-nonpolio entero combination. Routine immunization in Nigeria is low and in spite of the increase in the frequency of supplemental immunizations, a lot of children are still inadequately immunized, which may be the reason for our observation in this study. Although we are not dealing with a case of circulating vaccine derived poliovirus (cVDPV) yet, if the above condition persists, the advent of cVDVP may not be too far. There is therefore the need to maintain a high quality mass immunization and sustained routine immunization.