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Determination of the G and P types of previously nontypeable rotavirus strains from the African Rotavirus Network, 1996-2004: Identification of unusual G types

Determination of the G and P types of previously nontypeable rotavirus strains from the African Rotavirus Network, 1996-2004: Identification of unusual G types

Esona, M.D.; Steele, A.D.; Kerin, T.; Armah, G.E.; Peenze, I.; Geyer, A.; Page, N.; Nyangao, J.; Agbaya, V.A.; Trabelsi, A.; Tsion, B.; Aminu, M.; Sebunya, T.; Dewar, J.; Glass, R.; Gentsch, J.
URI: http://197.255.68.203/handle/123456789/1610
Date: 2010

Abstract:

A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.

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  • Electron Microscopy Department [77]
    The main research focus of the Electron Microscopy and Histopathology Department in the past 20 years has been in the areas of enteric diarrhoeas with special emphasis on rotavirus. Through its diarrhoea surveillance studies, the Department has helped to firmly establish rotaviruses as a major cause of diarrhoea in children, and document the circulation of unusual rotavirus genotypes in Ghana. The Department has also recently expanded its diagnostic repertoire to include the identification and characterization of noroviruses, astroviruses, and other enteric viruses.

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