|Title:||The development and validation of an enzyme linked immunosorbent assay for malaria|
|Keywords:||EMTREE medical terms: blood and hemopoietic system; cytology; diagnosis; enzyme immunoassay; immunofluorescence; malaria; methodology; protozoon; serodiagnosis|
MeSH: Antibodies; Caucasoid Race; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Human; Malaria; Negroid Race; Nigeria; Plasmodium; Plasmodium falciparum; Plasmodium vivax; Support, Non-U.S. Gov't
|Publisher:||Tropenmedizin Und Parasitologie|
|Citation:||Quakyi, I. A. (1980). The development and validation of an enzyme linked immunosorbent assay for malaria. Tropenmedizin Und Parasitologie, 31(3), 325-333|
|Abstract:||Vaccines for P. falciparum will need to contain both T- and B-cell epitopes. Conserved epitopes are the most desirable, but they are often poorly immunogenic. The major merozoite surface protein 1 (MSP-1) is currently a leading vaccine candidate antigen. In this study, six peptides from conserved or partly conserved regions of MSP-1 were evaluated for immunogenicity in B10 congenic mice. Following immunization with the peptides, murine T cells were tested for the ability to proliferate in vitro and antibody responses to MSP-1 were evaluated in vivo. The results showed that one highly conserved sequence (MSP-1#1, VTHESYQELVKKLEALEDAV; located at amino acid positions 20 to 39) and one partly conserved sequence (MSP-1#23, GLFHKEKMILNEEEITTKGA; located at positions 44 to 63) contained both T- and B-cell epitopes. Immunization of mice with these peptides resulted in T-cell proliferation and enhanced production of antibody to MSP-1 upon exposure to merozoites. MSP-1#1 stimulated T-cell responses in three of the six strains of mice evaluated, whereas MSP-1#23 was immunogenic in only one strain. Immunization with the other four peptides resulted in T-cell responses to the peptides, but none of the resulting peptide-specific T cells recognized native MSP-1. These results demonstrate that two sequences located in the N terminus of MSP-1 can induce T- and B-cell responses following immunization in a murine model. Clearly, these sequences merit further consideration for inclusion in a vaccine for malaria.|
|Appears in Collections:||School of Public Health 9|
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