Short time incubation at low temperature for retrieval of some antigens from formalin-fixed and paraffin-embedded tissues

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dc.contributor.author Seidu, M.A.
dc.contributor.author Adams, A.R.
dc.contributor.author Gyasi, R.K.
dc.contributor.author Tettey, Y.
dc.contributor.author Wiredu, E.K.
dc.date.accessioned 2013-06-20T10:21:10Z
dc.date.accessioned 2017-10-19T12:41:34Z
dc.date.available 2013-06-20T10:21:10Z
dc.date.available 2017-10-19T12:41:34Z
dc.date.issued 2012-07
dc.identifier.citation Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., & Wiredu, E. K. (2012). Short time incubation at low temperature for retrieval of some antigens from formalin-fixed and paraffin-embedded tissues. Journal of Histotechnology, 35(2), 74-79. en_US
dc.identifier.issn 01478885
dc.identifier.uri http://197.255.68.203/handle/123456789/3718
dc.description.abstract Antibody manufacturers often recommend antigen retrieval for formalin-fixed paraffin-embedded tissue sections before immunohistochemical staining. The most commonly used methods involve high temperatures (90-120°C) in buffers. Low-temperature retrieval methods allow the use of tissue flotation baths but their incubation times are often long. This study investigated retrieval of some antigens at low temperature (65°C) for 10 minutes in citrate buffer (pH 6.0) using a tissue flotation bath. Sections were obtained from two tissue specimens, breast and O. volvulus nodules, which were formalin-fixed and paraffin-embedded. The antigens of lactate dehydrogenase, pyruvate dehydrogenase kinase-2, malic enzyme-1, succinate dehydrogenase-B, glucose-6-phosphate dehydrogenase, alpha/beta-hydrolase-4, estrogen receptors (ER), and progesterone receptors (PR) were retrieved at low temperature and in the cases of ER and PR, were also retrieved at high temperature (12°C), using a pressure cooker, for comparison. All the retrievals were carried out in citrate buffer, pH 6.0. The results showed that all the enzymes scored 2+ (i.e. a strong reaction) in staining intensity and the staining for ER and PR were similar for both methods (2+). In immunohistochemical staining, the most important stage is antigen retrieval. The low-temperature antigen retrieval in citrate buffer at pH 6.0 provided staining intensities comparable to those by high-temperature methods. The results indicate that low-temperature antigen retrieval is cost-effective, rapid, and reliable. © National Society for Histotechnology 2012. en_US
dc.language.iso en en_US
dc.subject Antibodies; Epitopes; Flotation-bath; Pressure cooker en_US
dc.title Short time incubation at low temperature for retrieval of some antigens from formalin-fixed and paraffin-embedded tissues en_US
dc.type Article en_US


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