Bulk‐up synchronization of successive larval cohorts of Anopheles gambiae and Anopheles coluzzii through temperature reduction at early larval stages: efect on emergence rate, body size and mating success

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dc.contributor.author Zubair, Q.
dc.contributor.author Matthews, H.
dc.contributor.author Sougoufara, S.
dc.contributor.author Mujeeb, F.
dc.contributor.author Ashall, S.
dc.contributor.author Aboagye‑Antwi, F.
dc.contributor.author Tripet, F.
dc.date.accessioned 2021-09-22T14:58:11Z
dc.date.available 2021-09-22T14:58:11Z
dc.date.issued 2021
dc.identifier.citation https://doi.org/10.1186/s12936-021-03602-8 en_US
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/36743
dc.description.abstract Background: Malaria persists as a huge medical and economic burden. Although the number of cases and death rates have reduced in recent years, novel interventions are a necessity if such gains are to be maintained. Alterna‑ tive methods to target mosquito vector populations that involve the release of large numbers genetically modifed mosquitoes are in development. However, their successful introduction will require innovative strategies to bulk-up mosquito numbers and improve mass rearing protocols for Anopheles mosquitoes. Methods: The relationship between mosquito aquatic stage development and temperature was exploited so that multiple cohorts of mosquitoes, from separate egg batches, could be synchronized to ‘bulk-up’ the number of mos‑ quitoes released. First instar larvae were separated into two cohorts: the frst, maintained under standard insectary conditions at 27o C, the second subjected to an initial 5-day cooling period at 19o C. Results: Cooling of 1st instars slowed the mean emergence times of Anopheles coluzzii and Anopheles gambiae by 2.4 and 3.5 days, respectively, compared to their 27o C counterparts. Pupation and emergence rates were good (>85%) in all conditions. Temperature adjustment had no effect on mosquito sex ratio and adult fitness parameters such as body size and mating success. Conclusions: Bulk-up larval synchronization is a simple method allowing more operational fexibility in mosquito production towards mark-release-recapture studies and mass release interventions en_US
dc.description.sponsorship This work was funded by Target Malaria, a research consortium, which receives core funding from the Bill & Melinda Gates Foundation and from the Open Philanthropy Project Fund, an advised fund of Silicon Valley Community Foundation. These funding bodies have had no direct role in the design of the study nor in the collection, analysis, interpretation of data and in the writing of the manuscript. en_US
dc.language.iso en en_US
dc.publisher Malaria Journal en_US
dc.subject Anopheles gambiae en_US
dc.subject Anopheles coluzzii en_US
dc.subject Larvae development en_US
dc.subject Mass rearing en_US
dc.subject Mosquito release programmes en_US
dc.subject Mark release recapture studies en_US
dc.title Bulk‐up synchronization of successive larval cohorts of Anopheles gambiae and Anopheles coluzzii through temperature reduction at early larval stages: efect on emergence rate, body size and mating success en_US
dc.type Article en_US


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