Lack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patients

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dc.contributor.author Käser, M.
dc.contributor.author Gutmann, O.
dc.contributor.author Hauser, J.
dc.contributor.author Stinear, T.
dc.contributor.author Cole, S.
dc.contributor.author Yeboah-Manu, D.
dc.contributor.author Dernick, G.
dc.contributor.author Certa, U.
dc.contributor.author Pluschke, G.
dc.date.accessioned 2013-06-19T09:50:01Z
dc.date.accessioned 2017-10-16T13:06:41Z
dc.date.available 2013-06-19T09:50:01Z
dc.date.available 2017-10-16T13:06:41Z
dc.date.issued 2009
dc.identifier.citation Käser, M., Gutmann, O., Hauser, J., Stinear, T., Cole, S., Yeboah-Manu, D., . . . Pluschke, G. (2009). Lack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patients. Journal of Clinical Microbiology, 47(11), 3640-3646. en_US
dc.identifier.uri http://197.255.68.203/handle/123456789/3617
dc.description.abstract Mycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for microepidemiological studies aiming to characterize transmission pathways. In a previous comparative genomic hybridization analysis with a microarray covering part of the M. ulcerans genome, we have found extensive insertional-deletional sequence polymorphisms among M. ulcerans isolates of diverse geographic origins that allowed us to distinguish between strains coming from different continents. Since large numbers of insertion sequences are spread over the genome of African M. ulcerans strains, we reasoned that these may drive large sequence polymorphisms in otherwise clonal local mycobacterial populations. In this study, we used a printed DNA microarray covering the whole genome of the Ghanaian M. ulcerans reference strain Agy99 for comparative genomic hybridization. The assay identified multiple regions of difference when DNA of a Japanese M. ulcerans strain was analyzed. In contrast, not a single insertional-deletional genomic variation was found within a panel of disease isolates coming from an area of Ghana where Buruli ulcer is endemic. These results indicate that, despite the expectations deduced from other mycobacterial pathogens, only analyses of single nucleotide polymorphisms will have the potential to differentiate local populations of M. ulcerans. en_US
dc.language.iso en en_US
dc.publisher Journal of Clinical Microbiology en_US
dc.title Lack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patients en_US
dc.type Article en_US


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  • Bacteriology Department [97]
    The Bacteriology Department aims to improve the quality of life first for Ghanaians and the world at large by conducting research into bacterial diseases of public health importance to Ghana and globally. In addition to working on enteric pathogens and sexually transmitted diseases, the department’s current main focus is on the two most important mycobacterial diseases of public health importance to Ghana, namely Buruli ulcer (BU) and tuberculosis (TB).

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