dc.contributor.author |
Fordjuor, G. |
|
dc.contributor.author |
Kin, S.II. |
|
dc.contributor.author |
Park, C.Y. |
|
dc.contributor.author |
Lee, J.H. |
|
dc.contributor.author |
Lee, J.S. |
|
dc.contributor.author |
Lee, J.E. |
|
dc.date.accessioned |
2019-11-25T09:15:16Z |
|
dc.date.available |
2019-11-25T09:15:16Z |
|
dc.date.issued |
2019-11-08 |
|
dc.identifier.other |
https://doi.org/10.1186/s12886-019-1228-5 |
|
dc.identifier.uri |
http://ugspace.ug.edu.gh/handle/123456789/33802 |
|
dc.description |
Research Article |
en_US |
dc.description.abstract |
Background: To investigate the cytotoxicities of the topical ocular dual-action anti-allergic agents (alcaftadine
0.25%, bepotastine besilate 1.5%, and olopatadine HCL 0.1%) on human corneal epithelial cells (HCECs) and their
anti-allergic effects on cultured conjunctival epithelial cells.
Methods: A Methylthiazolyltetrazolium(MTT)-based calorimetric assay was used to assess cytotoxicities using HCECs
at concentrations of 10, 20 or 30% for exposure durations of 30 min, 1 h, 2 h, 12 h or 24 h. Cellular morphologies
were evaluated by inverted phase-contrast and electron microscopy. Wound widths were measured 2 h, 18 h, or 24
h after confluent HCECs monolayers were scratched. Realtime PCR was used to quantify anti-allergic effects on
cultured human conjunctival cells, in which allergic reactions were induced by treating them with Aspergillus
antigen.
Results: Cell viabilities decreased in time- and concentration-dependent manners. Cells were detached from dishes
and showed microvilli loss, cytoplasmic vacuoles, and nuclear condensation when exposed to antiallergic agents;
alcaftadine was found to be least cytotoxic. Alcaftadine treated HCECs monolayers showed the best wound healing
followed by bepotastine and olopatadine (p < 0.0001). All agents significantly reduced the gene expressions of
allergic cytokines (IL-5, IL-25, eotaxin, thymus and activation-regulated chemokine, and thymic stromal
lymphopoietin) and alcaftadine had the greatest effect (p < 0.0001 in all cases).
Conclusions: Alcaftadine seems to have less side effects and better therapeutic effects than the other two antiallergic
agents tested. It may be more beneficial to use less toxic agents for patients with ocular surface risk factors
or presumed symptoms of toxicity. |
en_US |
dc.description.sponsorship |
Research Institute for Convergence of
biomedical science and technology Grant (30–2016-017), Pusan National
University Yangsan Hospital. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
BMC Ophthalmology |
en_US |
dc.relation.ispartofseries |
217;2019 |
|
dc.subject |
Allergic conjunctivitis |
en_US |
dc.subject |
Cytotoxicity |
en_US |
dc.subject |
Topical anti-allergic agents |
en_US |
dc.subject |
human corneal epithelial cells (HCECs) |
en_US |
dc.title |
Comparison of cytotoxicities and antiallergic effects of topical ocular dual-action anti-allergic agents |
en_US |
dc.type |
Article |
en_US |