Re-evaluation of the plasma Creatinine Determination by the Acidification method

Show simple item record Arthur, S.K. Nyarko, A. 2019-11-08T16:22:58Z 2019-11-08T16:22:58Z 1988-03
dc.description Journal Article en_US
dc.description.abstract The acidification method for measuring plasma creatinine has been used to determine the role played by bilirubin and ingested drugs. as interfering substances in the Jaffe reaction. This work was made necessary because of earlier reports by some authors that the acidification method for the determination of creatinine is non-specific. The reason given for the non-specificity of this method is that acidification does not only destroy creatinine chromogen but non-creatinine chromogen as well, chief among them being bilirubin. In this study it was evident that the interfering role of bilirubin is nil; it has no influence on creatinine levels determined by the acidification method, if plasma samples are deproteinized prior to analysis. This fact is being particularly emphasised as it has not been emphasised in the literature. It was also evident that over a wide pH range (4.5 - 12) bilirubin chromogen measured by the Jaffe reaction remained fairly stable and contributed insignificantly to the creatinine level in solution. Plasma creatinine was completely destroyed after acidication. Plasma creatinine levels in Ghanaians measured by our method is not significantly different from the true plasma creatinine level in Caucasians measured by more sophisticated methods. The modified acidification method is therefore recommended as a simple, inexpensive and practical method for the routine determination of plasma creatinine. en_US
dc.description.sponsorship University of Ghana Medical School en_US
dc.language.iso en en_US
dc.publisher Ghana Medical Journal en_US
dc.relation.ispartofseries 22;1
dc.subject Creatinine determination en_US
dc.subject non-creatinine chromogen en_US
dc.subject Ghana en_US
dc.subject plasma creatinine en_US
dc.title Re-evaluation of the plasma Creatinine Determination by the Acidification method en_US
dc.type Article en_US

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