A shared Asian origin of the triple-mutant DHFR allele in plasmodium falciparum from sites across Africa.

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dc.contributor.author Maiga, O.
dc.contributor.author Djimde, A.A.
dc.contributor.author Hubert, V.
dc.contributor.author Renard, E.
dc.contributor.author Aubouy, A.
dc.contributor.author Kironde, F.
dc.contributor.author Nsimba, B.
dc.contributor.author Koram, K.A.
dc.contributor.author Doumbo, O.K.
dc.contributor.author Le Bras, J.
dc.contributor.author Clain, J.
dc.date.accessioned 2013-06-17T15:38:14Z
dc.date.accessioned 2017-10-16T13:08:09Z
dc.date.available 2013-06-17T15:38:14Z
dc.date.available 2017-10-16T13:08:09Z
dc.date.issued 2007-07-01
dc.identifier.citation Maïga, O., Djimdé, A. A., Hubert, V., Renard, E., Aubouy, A., Kironde, F., . . . Clain, J. (2007). A shared Asian origin of the triple-mutant dhfr allele in plasmodium falciparum from sites across africa. Journal of Infectious Diseases, 196(1), 165-172. en_US
dc.identifier.issn 00221899
dc.description.abstract Background. Usefulness of sulfadoxine-pyrimethamine as first-line therapy for uncomplicated Plasmodium falciparum malaria and intermittent preventive treatment in pregnancy throughout sub-Saharan Africa is compromised by the spread of dhfr alleles associated with pyrimethamine resistance. A predominant haplotype associated with the N51I+C59R+S108N triple-mutant dhfr allele has been reported recently in 4 African countries. A more comprehensive picture of the evolution of this mutant allele in Africa is lacking. Methods. Seventy-five P. falciparum isolates carrying the wild-type dhfr allele and 204 carrying the triple-mutant dhfr allele from 11 African countries were selected. The genetic diversity of the chromosomes bearing these alleles was analyzed with 4 microsatellite markers closely linked to the dhfr gene. Results. Seventy-three different 4-locus haplotypes carrying the wild-type dhfr allele were found. By contrast, 175 (85%) of 204 isolates carrying the triple-mutant dhfr allele shared a unique haplotype, identical to the one identified in Thailand. For the remaining triple-mutant isolates and one isolate with the quadruple-mutant dhfr allele (N51I+C59R+S108N+I164L), haplotypes were closely related to the predominant haplotype by mutation or recombination. Conclusions. Migration of parasites carrying an ancestral triple-mutant dhfr allele drives the spread of dhfr alleles associated with pyrimethamine resistance throughout West and Central Africa. © 2007 by the Infectious Diseases Society of America. All rights reserved. en_US
dc.subject EMTREE medical terms: adult; Africa; allele; article; Asian; chromosome; dhfr gene; female; gene; gene locus; gene mutation; genetic recombination; genetic variability; haplotype; heterozygote; human; infection prevention; major clinical study; malaria; male; microsatellite marker; molecular evolution; mutant; nonhuman; parasite isolation; Plasmodium falciparum; priority journal; protozoal genetics; Thailand; wild type en_US
dc.title A shared Asian origin of the triple-mutant DHFR allele in plasmodium falciparum from sites across Africa. en_US
dc.type Article en_US

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    The Epidemiology Department contributes to the mission of the institute through basic and applied epidemiological research on, but not limited to, malaria and other diseases of public health importance. It is also home to the Social Science Unit of the Institute, including the Health Support Centre for HIV/AIDS and other communicable and noncommunicable health problems.

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