Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test

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dc.contributor.author Njiru, Z.K.
dc.contributor.author Yeboah-Manu, D.
dc.contributor.author Stinear, T.P.
dc.contributor.author Fyfe, J.A.M.
dc.date.accessioned 2013-06-17T08:43:29Z
dc.date.accessioned 2017-10-16T13:19:28Z
dc.date.available 2013-06-17T08:43:29Z
dc.date.available 2017-10-16T13:19:28Z
dc.date.issued 2012
dc.identifier.citation Njiru, Z. K., Yeboah-Manu, D., Stinear, T. P., & Fyfe, J. A. M. (2012). Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test. Journal of Clinical Microbiology, 50(5), 1737-1741. en_US
dc.identifier.uri http://197.255.68.203/handle/123456789/3245
dc.description.abstract This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions en_US
dc.language.iso en en_US
dc.publisher Journal of Clinical Microbiology en_US
dc.title Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test en_US
dc.type Article en_US


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  • Bacteriology Department [105]
    The Bacteriology Department aims to improve the quality of life first for Ghanaians and the world at large by conducting research into bacterial diseases of public health importance to Ghana and globally. In addition to working on enteric pathogens and sexually transmitted diseases, the department’s current main focus is on the two most important mycobacterial diseases of public health importance to Ghana, namely Buruli ulcer (BU) and tuberculosis (TB).

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