Whole genome characterization and evolutionary analysis of OP354-like P[8] Rotavirus A strains isolated from Ghanaian children with diarrhoea

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dc.contributor.author Damanka, S.A.
dc.contributor.author Kwofie, S.
dc.contributor.author Dennis, F.E.
dc.contributor.author Lartey, B.L.
dc.contributor.author Agbemabiese, C.A.
dc.contributor.author Doan, Y.H.
dc.contributor.author Adiku, T.K.
dc.contributor.author Katayama, K.
dc.contributor.author Enweronu-Laryea, C.C.
dc.contributor.author Armah, G.E.
dc.date.accessioned 2019-09-13T16:35:34Z
dc.date.available 2019-09-13T16:35:34Z
dc.date.issued 2019-05-30
dc.identifier.other https://doi.org/10.1371/journal. pone.0218348
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/32185
dc.description Research Article en_US
dc.description.abstract In 2010, the rare OP354-like P[8]b rotavirus subtype was detected in children less than 2 years old in Ghana. In this follow-up study, to provide insight into the evolutionary history of the genome of Ghanaian P[8]b strains RVA/Human-wt/GHA/GHDC949/2010/G9P[8] and RVA/Human-wt/GHA/GHM0094/2010/G9P[8] detected in an infant and a 7-month old child hospitalised for acute gastroenteritis, we sequenced the complete genome using both Sanger sequencing and Illumina MiSeq technology followed by phylogenetic analysis of the near-full length sequences. Both strains possessed the Wa-like/genotype 1 constellation G9P[8]b-I1-R1-C1-M1-A1-N1-T1-E1-H1. Sequence comparison and phylogenetic inference showed that both strains were identical at the lineage level throughout the 11 genome segments. Their VP7 sequences belonged to the major sub-lineage of the G9-lineage III whereas their VP4 sequences belonged to P[8]b cluster I. The VP7 and VP4 genes of the study strains were closely related to a Senegalese G9P[8]b strain detected in 2009. In the remaining nine genome segments, both strains consistently clustered together with Wa-like RVA strains possessing either P[8]a or P[8]b mostly of African RVA origin. The introduction of a P[8]b subtype VP4 gene into the stable Wa-like strain backbone may result in strains that might propagate easily in the human population, with a potential to become an important public health concern, especially because it is not certain if the monovalent rotavirus vaccine (Rotarix) used in Ghana will be efficacious against such strains. Our analysis of the full genomes of GHM0094 and GHDC949 adds to knowledge of the genetic make-up and evolutionary dynamics of P[8]b rotavirus strains en_US
dc.language.iso en en_US
dc.publisher PLOS ONE en_US
dc.relation.ispartofseries 14;6
dc.subject Genome en_US
dc.subject evolutionary analysis en_US
dc.subject Rotavirus en_US
dc.subject diarrhoea en_US
dc.subject Ghana en_US
dc.title Whole genome characterization and evolutionary analysis of OP354-like P[8] Rotavirus A strains isolated from Ghanaian children with diarrhoea en_US
dc.type Article en_US

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  • Electron Microscopy Department [67]
    The main research focus of the Electron Microscopy and Histopathology Department in the past 20 years has been in the areas of enteric diarrhoeas with special emphasis on rotavirus. Through its diarrhoea surveillance studies, the Department has helped to firmly establish rotaviruses as a major cause of diarrhoea in children, and document the circulation of unusual rotavirus genotypes in Ghana. The Department has also recently expanded its diagnostic repertoire to include the identification and characterization of noroviruses, astroviruses, and other enteric viruses.

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