Point of Care Diagnosis of Multiple Schistosome Parasites: Species-Specific DNA Detection in Urine by Loop-Mediated Isothermal Amplification (LAMP)

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dc.contributor.author Lodh, N.
dc.contributor.author Mikita, K.
dc.contributor.author Bosompem, K.M.
dc.contributor.author Anyan, W.K.
dc.contributor.author Quartey, J.K.
dc.contributor.author Otchere, J.
dc.contributor.author Shiff, C.J.
dc.date.accessioned 2019-07-31T11:42:38Z
dc.date.available 2019-07-31T11:42:38Z
dc.date.issued 2017-06
dc.identifier.issn Vol.173
dc.identifier.other DOI: 10.1016/j.actatropica.2017.06.015
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/31898
dc.description.abstract Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use. It is known and regularly demonstrated that praziquantel does not always eliminate an infection so in spite of the successes of control programs a residual of the reservoir survives to re-infect snails. The issue of diagnostic sensitivity becomes more critical in the assessment of program effectiveness. While serology, such as antigen capture tests might improve sensitivity, it has been shown that the presence of species-specific DNA fragments will indicate, most effectively, the presence of active parasites. Polymerase chain reaction (PCR) can amplify and detect DNA from urine residue captured on Whatman No. 3 filter paper that is dried after filtration. Previously we have detected S. mansoni and S. haematobium parasite specific small repeat DNA fragment from filtered urine on filter paper by PCR. In the current study, we assessed the efficacy of detection of 86 urine samples for either or both schistosome parasites by PCR and loop-mediated isothermal amplification (LAMP) that were collected from a low to moderate transmission area in Ghana. Two different DNA extraction methods, standard extraction kit and field usable LAMP-PURE kit were also evaluated by PCR and LAMP amplification. With S. haematobium LAMP amplification for both extractions showed similar sensitivity and specificity when compared with PCR amplification (100%) verified by gel electrophoresis. For S. mansoni sensitivity was highest for LAMP amplification (100%) for standard extraction than PCR and LAMP with LAMP-PURE (99% and 94%). The LAMP-PURE extraction produced false negatives, which require further investigation for this field usable extraction kit. Overall high positive and negative predictive values (90% − 100%) for both species demonstrated a highly robust approach. The LAMP approach is close to point of care use and equally sensitive and specific to detection of species-specific DNA by PCR. LAMP can be an effective means to detect low intensity infection due to its simplicity and minimal DNA extraction requirement. This will enhance the effectiveness of surveillance and MDA control programs of schistosomiasis. en_US
dc.language.iso en en_US
dc.publisher Acta Tropica en_US
dc.subject LAMP en_US
dc.subject LAMP-PURE en_US
dc.subject PCR en_US
dc.subject Repeat DNA en_US
dc.subject Schistosomiasis en_US
dc.subject Urine en_US
dc.title Point of Care Diagnosis of Multiple Schistosome Parasites: Species-Specific DNA Detection in Urine by Loop-Mediated Isothermal Amplification (LAMP) en_US
dc.type Article en_US

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  • Parasitology Department [275]
    The Department of Parasitology conducts research into parasitic diseases of public health importance with the overall goal of reducing their transmission and the heavy disease burden that they impose on affected populations. The Department maintains focus on parasitic diseases in general. These include major diseases such as malaria, and others listed under the Neglected Tropical Diseases (NTD) control initiative such as, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminthiasis, trypanosomiasis and leishmaniasis.

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