Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria patients living in the Central Region of Ghana

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dc.contributor.author Acquah, F.K.
dc.contributor.author Obboh, F.K.
dc.contributor.author Asare, K.
dc.contributor.author Boampong, J.N.
dc.contributor.author Nuvor, S.V.
dc.contributor.author Singh, S.K.
dc.contributor.author Theisen, M.
dc.contributor.author Williamson, K.C.
dc.contributor.author Amoah, L.E.
dc.date.accessioned 2019-07-23T11:53:52Z
dc.date.available 2019-07-23T11:53:52Z
dc.date.issued 2017
dc.identifier.other vol. 16(1)
dc.identifier.other DOI:10.1186/s12936-017-1955-0
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/31666
dc.description.abstract Background: Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Methods: Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Results: Pfs48/45.6C and Pfs230.C0 were successfully produced in L. lactis in the absence of a fusion partner using a simplified purification scheme. Seroprevalence for L. lactis-produced Pfs48/45.6C and Pfs230.C0 in the study population was 74.7 and 72.8%, respectively. Conclusions: A significant age-dependent increase in antibody titers was observed, which suggests a vaccine targeting these antigens could be boosted during a natural infection in the field. © 2017 The Author(s). en_US
dc.language.iso en en_US
dc.publisher Malaria Journal en_US
dc.subject Lactococcus lactis; Malaria; Pfs230; Pfs48/45; Seropositive; Transmission-blocking en_US
dc.title Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria patients living in the Central Region of Ghana en_US
dc.type Article en_US


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  • Clinical Pathology Department [56]
    The Clinical Pathology Department conducts research that contributes to intervention strategies and safeguards of public health in Ghana. In view of this the Department carries out research into plant medicine development, drug interactions and regulation, bio-monitoring and prevention of poisoning from environmental toxicants (e.g. mycotoxins specifically aflatoxins and toxic heavy metals). The Department also provides specialized laboratory services in support of public health programmes.

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