Dynamics of anti-MSP3 and Pfs230 antibody responses and multiplicity of infection in asymptomatic children from southern Ghana

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dc.contributor.author Amoah, L.E.
dc.contributor.author Acquah, F.K.
dc.contributor.author Ayanful-Torgby, R.
dc.contributor.author Oppong, A.
dc.contributor.author Abankwa, J.
dc.contributor.author Obboh, E.K.
dc.contributor.author Singh, S.K.
dc.contributor.author Theisen, M.
dc.date.accessioned 2019-07-15T12:12:25Z
dc.date.available 2019-07-15T12:12:25Z
dc.date.issued 2018-01
dc.identifier.other https://doi.org/10.1186/s13071-017-2607-5
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/31455
dc.description.abstract Background During a Plasmodium infection, exposure of human host immune cells to both the asexual and the sexual stages of the parasite elicit immune responses. These responses may be protective and prevent the development of high parasitaemia and its associated clinical symptoms, or block the transmission of malaria to an uninfected person. This study aimed at examining the dynamics of naturally acquired immune responses against the asexual and sexual forms of Plasmodium falciparum as well as assessing differences in the multiplicity of infection (MOI) in asymptomatic Ghanaian children living in two communities with varying malaria transmission intensities. Methods School children aged between 6 and 12 years were recruited from Obom, a high malaria prevalence setting and Abura, a low malaria prevalence setting and enrolled in monthly multiple cross sectional surveys between February and May 2015. Filter paper blood blots (DBS) as well as thick and thin blood smears were made from finger-pricked blood at each visit. Plasmodium falciparum parasite prevalence was determined by microscopy and PCR. Serum eluted from the DBS were used to assess anti-Pfs230 (sexual stage) and anti-MSP3 (asexual stage) antibody levels using indirect ELISA and DNA extracted from the DBS used to assess MOI. Results Malaria parasite point prevalence and MOI throughout the study was higher in Obom than Abura. The trend of parasite prevalence estimated by microscopy was similar to that determined by PCR in Obom but not in Abura. The trend of MSP3 antibody seroprevalence followed that of PCR-estimated parasite prevalence in Obom, while in Abura the trend of Pfs230 antibody seroprevalence followed that of PCR-estimated parasite prevalence. Conclusions Microscopy can more accurately predict changes in parasite prevalence in high transmission settings than low transmission settings. In high transmission settings, P. falciparum parasite prevalence can predict antibody seroprevalence to MSP3, whilst in low transmission settings, seroprevalence against Pfs230 may be a useful predictor of parasite prevalence. en_US
dc.language.iso en en_US
dc.publisher Parasites & vectors en_US
dc.subject Malaria en_US
dc.subject Transmission en_US
dc.subject Pfs230 en_US
dc.subject MSP3 en_US
dc.subject Seroprevalence en_US
dc.subject Antibodies en_US
dc.title Dynamics of anti-MSP3 and Pfs230 antibody responses and multiplicity of infection in asymptomatic children from southern Ghana en_US
dc.type Article en_US

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  • Parasitology Department [253]
    The Department of Parasitology conducts research into parasitic diseases of public health importance with the overall goal of reducing their transmission and the heavy disease burden that they impose on affected populations. The Department maintains focus on parasitic diseases in general. These include major diseases such as malaria, and others listed under the Neglected Tropical Diseases (NTD) control initiative such as, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminthiasis, trypanosomiasis and leishmaniasis.

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