Second-line anti-tuberculosis drug resistance testing in Ghana identifies the first extensively drug-resistant tuberculosis case

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dc.contributor.author Osei-Wusu, S.
dc.contributor.author Omari, M.A.
dc.contributor.author Asante-Poku, A.
dc.contributor.author Otchere, I.D.
dc.contributor.author Asare, P.
dc.contributor.author Forson, A.
dc.contributor.author Otu, J.
dc.contributor.author Antonio, M.
dc.contributor.author Yeboah-Manu, D.
dc.date.accessioned 2019-07-11T16:12:28Z
dc.date.available 2019-07-11T16:12:28Z
dc.date.issued 2018-02
dc.identifier.other doi: 10.2147/IDR.S152720
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/31404
dc.description.abstract Background Drug resistance surveillance is crucial for tuberculosis (TB) control. Therefore, our goal was to determine the prevalence of second-line anti-TB drug resistance among diverse primary drug-resistant Mycobacterium tuberculosis complex (MTBC) isolates in Ghana. Materials and methods One hundred and seventeen MTBC isolates with varying first-line drug resistance were analyzed. Additional resistance to second-line anti-TB drugs (streptomycin [STR], amikacin [AMK] and moxifloxacin [MOX]) was profiled using the Etest and GenoType MTBDRsl version 2.0. Genes associated with resistance to AMK and MOX (gyrA, gyrB, eis, rrs, tap, whiB7 and tlyA) were then analyzed for mutation. Results Thirty-seven (31.9%) isolates had minimum inhibitory concentration (MIC) values ≥2 µg/mL against STR while 12 (10.3%) isolates had MIC values ≥1 µg/mL for AMK. Only one multidrug-resistant (MDR) isolate (Isolate ID: TB/Nm 919) had an MIC value of ≥0.125 µg/mL for MOX (MIC = 3 µg/mL). This isolate also had the highest MIC value for AMK (MIC = 16 µg/mL) and was confirmed as resistant to AMK and MOX by the line probe assay GenoType MTBDRsl version 2.0. Mutations associated with the resistance were: gyrA (G88C) and rrs (A514C and A1401G). Conclusion Our findings suggest the need to include routine second-line anti-TB drug susceptibility testing of MDR/rifampicin-resistant isolates in our diagnostic algorithm. en_US
dc.language.iso en en_US
dc.publisher Infection and Drug Resistance en_US
dc.subject Tuberculosis en_US
dc.subject Drug resistance en_US
dc.subject Diagnosis en_US
dc.subject Ghana en_US
dc.subject XDR en_US
dc.title Second-line anti-tuberculosis drug resistance testing in Ghana identifies the first extensively drug-resistant tuberculosis case en_US
dc.type Article en_US


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  • Bacteriology Department [105]
    The Bacteriology Department aims to improve the quality of life first for Ghanaians and the world at large by conducting research into bacterial diseases of public health importance to Ghana and globally. In addition to working on enteric pathogens and sexually transmitted diseases, the department’s current main focus is on the two most important mycobacterial diseases of public health importance to Ghana, namely Buruli ulcer (BU) and tuberculosis (TB).

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