A monoclonal antibody-based dipstick assay for diagnosis of urinary schistosomiasis.

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dc.contributor.author Bosompem, K.M.
dc.contributor.author Ayi, I.
dc.contributor.author Anyan, W.K.
dc.contributor.author Arishima, T.
dc.contributor.author Nkrumah, F.K.
dc.contributor.author Kojima, S.
dc.date.accessioned 2013-06-12T11:52:45Z
dc.date.accessioned 2017-10-16T12:58:02Z
dc.date.available 2013-06-12T11:52:45Z
dc.date.available 2017-10-16T12:58:02Z
dc.date.issued 1997
dc.identifier.citation Bosompem, K. M., Ayi, I., Anyan, W. K., Arishima, T., Nkrumah, F. K., & Kojima, S. (1997). A monoclonal antibody-based dipstick assay for diagnosis of urinary schistosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91(5), 554-556. en_US
dc.identifier.uri http://197.255.68.203/handle/123456789/3090
dc.description.abstract A Schistosoma haematobium species-specific mouse immunoglobulin (Ig) G1 monoclonal antibody (mab) Sh2/15.F that bound a 29 kDa peptide was utilized to develop a membrane-based dipstick enzyme-linked immunosorbent assay for specific diagnosis of urinary schistosomiasis. Strips of polyvinylidene difluoride membrane were wetted with methanol and stored in distilled water. The strips were used to capture urinary antigens which were then revealed by incubation in a mixture of specific mab and peroxidase-conjugated goat anti-mouse IgG. The assay correctly identified (87%) of egg-negative control individuals and (98%) of parasitologically confirmed cases including all of 6 individuals treated with praziquantel (40 mg/kg) but not cured. Also, the assay detected S. haematobium antigens in the urine of 3 individuals from whom 2 specimens had to be examined microscopically to confirm infection, thus suggesting that the mab detection method may have greater sensitivity than microscopy. en_US
dc.language.iso en en_US
dc.publisher Transactions of the Royal Society of Tropical Medicine and Hygiene en_US
dc.subject Schistosomiasis en_US
dc.subject Schistosoma haematobium en_US
dc.subject Diagnosis en_US
dc.subject Dipstick test en_US
dc.subject Enzyme-linked immunosorbent assay en_US
dc.title A monoclonal antibody-based dipstick assay for diagnosis of urinary schistosomiasis. en_US
dc.type Article en_US


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  • Clinical Pathology Department [55]
    The Clinical Pathology Department conducts research that contributes to intervention strategies and safeguards of public health in Ghana. In view of this the Department carries out research into plant medicine development, drug interactions and regulation, bio-monitoring and prevention of poisoning from environmental toxicants (e.g. mycotoxins specifically aflatoxins and toxic heavy metals). The Department also provides specialized laboratory services in support of public health programmes.

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