Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles

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dc.contributor.author Chabi, J.
dc.contributor.author Van’t Hof, A.
dc.contributor.author N’dri, L.K.
dc.contributor.author Datsomor, A.
dc.contributor.author Okyere, D.
dc.contributor.author Njoroge, H.
dc.contributor.author Pipini, D.
dc.contributor.author Hadi, M.P.
dc.contributor.author De Souza, D.K.
dc.contributor.author Suzuki, T.
dc.contributor.author Dadzie, S.K.
dc.contributor.author Jamet, H.P.
dc.date.accessioned 2019-05-16T16:42:39Z
dc.date.available 2019-05-16T16:42:39Z
dc.date.issued 2019-04
dc.identifier.citation Chabi J, Van’t Hof A, N’dri LK, Datsomor A, Okyere D, Njoroge H, et al. (2019) Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles. PLoS ONE 14(4): e0215669. https://doi.org/10.1371/journal.pone.0215669 en_US
dc.identifier.other https://doi.org/10.1371/journal.pone.0215669
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/30097
dc.description.abstract The Anopheles gambiae sensu lato species complex consists of a number of cryptic species with different habitats and behaviours. These morphologically indistinct species are identified by chromosome banding. Several molecular diagnostic techniques for distinguishing between An. coluzzii and An. gambiae are still under improvement. Although, the current SINE method for identification between An. coluzzii and An. gambiae works reliably, this study describes a refinement of the SINE method to increase sensitivity for identification of An. coluzzii, An. gambiae and An. arabiensis based on amplicon dissociation curve characteristics. Field-collected samples, laboratory-reared colonies and crossed specimens of the two species were used for the design of the protocol. An. gambiae, An. coluzzii, and hybrids of the two species were sampled from Ghana and An. arabiensis from Kenya. Samples were first characterised using conventional SINE PCR method, and further assayed using SYBR green, an intercalating fluorescent dye. The three species and hybrids were clearly differentiated using the melting temperature of the dissociation curves, with derivative peaks at 72˚C for An. arabiensis, 75˚C for An. gambiae and 86˚C for An. coluzzii. The hybrids (An. gambiae / An. coluzzii) showed both peaks. This work is the first to describe a SYBR green real time PCR method for the characterization of An. arabiensis, An. gambiae and An. coluz-zii and was purposely designed for basic melt-curve analysis (rather than high-resolution melt-curve) to allow it to be used on a wide range of real-time PCR machines. en_US
dc.language.iso en en_US
dc.publisher PLoS ONE en_US
dc.title Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles en_US
dc.type Article en_US


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  • Parasitology Department [244]
    The Department of Parasitology conducts research into parasitic diseases of public health importance with the overall goal of reducing their transmission and the heavy disease burden that they impose on affected populations. The Department maintains focus on parasitic diseases in general. These include major diseases such as malaria, and others listed under the Neglected Tropical Diseases (NTD) control initiative such as, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminthiasis, trypanosomiasis and leishmaniasis.

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