Comparative study of 2,4-D and Picloram on friable embryogenic calli and somatic embryos development in cassava (Manihot esculenta Crantz)

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dc.contributor.author Danso, K.E.
dc.contributor.author Elegba, W.
dc.contributor.author Oduro, V.
dc.contributor.author Kpentey, P.
dc.date.accessioned 2019-04-30T10:01:53Z
dc.date.available 2019-04-30T10:01:53Z
dc.date.issued 2010-01
dc.identifier.other Vol.10(2): pp 94-100
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/29677
dc.description.abstract A comparative study on the effect of 2,4-D and Picloram on primary and cyclic embryo production as well as plant conversion in three local accessions of cassava was conducted. Leaf lobe explants cultured on MS medium supplemented with 8 mg/l (2,4-D) or 16 mg/l (Picloram) induced the highest percentage embryogenic calli. On transfer to maturation medium (MS plus 0.1mg/l BAP), Picloram enhanced early embryo maturation (9 days), highest mean number of primary and cyclic embryo production (two-fold) than 2,4-D except in Wenchi and ADI 001 where leaf lobe explants cultured on MS medium supplemented with 20 mg/l Picloram did not develop embryogenic calli. Somatic embryos produced by Picloram induced calli had thick and elongated hypocotyls which were oriented horizontally on the adaxial surface of the calli while most of the 2,4-D induced embryos mostly had fascinated hypocotyls vertically on the uppers surface. Desiccation of Picloram-induced somatic embryos significantly (P≤0.05) improved plant conversion than 2,4-D- induced embryos. Also, pretreatment of 2,4-D or Picloram- induced embryos with low concentrations of abscisic acid (ABA) prior to desiccation enhanced higher frequency of germination of somatic embryos. This study has shown that Picloram enhances early maturation, high frequency embryo production as well as plant conversion, thus providing an alternative auxin for cassava somatic embryogenesis. © IJIB, All rights reserved. en_US
dc.language.iso en en_US
dc.publisher International Journal of Integrative Biology en_US
dc.subject 2,4-dichlorophenoxy acetic acid en_US
dc.subject Embryogenic calli en_US
dc.subject Picloram en_US
dc.subject Plant conversion en_US
dc.subject Somatic embryo en_US
dc.title Comparative study of 2,4-D and Picloram on friable embryogenic calli and somatic embryos development in cassava (Manihot esculenta Crantz) en_US
dc.type Article en_US


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