The middle to 3′ end of the HIV-1 Vif gene sequence is important for Vif biological activity and could be used for antisense oligonucleotide targets

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dc.contributor.author Barnor, J.S.
dc.contributor.author Miyano-Kurosaki, N.
dc.contributor.author Takaku, H.
dc.contributor.author Yamaguchi, K.
dc.contributor.author Sakamoto, A.
dc.contributor.author Ishikawa, K.
dc.contributor.author Yamamoto, N.
dc.contributor.author Osei-Kwasi, M.
dc.contributor.author Ofori-Adjei, D.
dc.date.accessioned 2019-03-21T08:43:05Z
dc.date.available 2019-03-21T08:43:05Z
dc.date.issued 2005-02
dc.identifier.other Vol. 24(10-12): pp 1745-61
dc.identifier.other https://doi.org/10.1080/10810730500265823
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/28750
dc.description.abstract □ The human immunodeficiency virus type-1 (HIV-1)-encoded Vif protein is essential for viral replication, virion production, and pathogenicity. HIV-1 Vif interacts with the endogenous human APOBEC3G protein (an mRNA editor) in target cells to prevent its encapsidation into virions. Some studies have established targets within the HIV-1 vif gene that are important for its biologic function; however, it is important to determine effective therapeutic targets in vif because of its critical role in HIV-1 infectivity and pathogenicity. The present study demonstrates that virions generated in transfected HeLa-CD4 + cells, especially from HIV-1 vif frame-shift mutant (3′-△vif; 5561-5849), were affected in splicing and had low infectivity in MT-4 cells. In addition, HIV-1 vif antisense RNA fragments constructed within the same region, notably the region spanning nucleic acid positions 5561-5705 (M-3′-AS), which corresponds to amino acid residues 96-144, significantly inhibited HIV-1 replication in MT-4 and reduced the HTV-1 vif mRNA transcripts and reporter gene (EGFP) expression. The generated virions showed low secondary infection in H9 cells. These data therefore suggest that the middle to the 3′ end of vif is important for its biological activity in the target cells. Copyright © Taylor & Francis Group, LLC. en_US
dc.language.iso en en_US
dc.publisher Nucleosides, Nucleotides and Nucleic Acids en_US
dc.subject Antisense RNA en_US
dc.subject HIV-1 vif gene en_US
dc.subject Inhibition of HIV-1 replication en_US
dc.subject Oligonucleotides en_US
dc.subject Vif biological activity en_US
dc.title The middle to 3′ end of the HIV-1 Vif gene sequence is important for Vif biological activity and could be used for antisense oligonucleotide targets en_US
dc.type Article en_US


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  • Virology Department [118]
    Most research activities involve the use of molecular methods such as regular PCR (PCR), quantitative PCR (Q-PCR), genomic sequence and analysis using different software, genetic engineering, probe hybridization techniques, biological and molecular cloning, evaluation of immune markers for laboratory diagnosis of infections, serological assays involving the use of rapid tests, ELISA-based evaluations and immunofluorescent assay techniques.

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