Respiratory syncytial virus genotypes circulating in urban Ghana: February to November 2006

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dc.contributor.author Obodai, E.
dc.contributor.author Asmah, R.
dc.contributor.author Boamah, I.
dc.contributor.author Goka, B.
dc.contributor.author Odoom, J.K.
dc.contributor.author Adiku, T.
dc.date.accessioned 2018-11-08T15:25:23Z
dc.date.available 2018-11-08T15:25:23Z
dc.date.issued 2014-10
dc.identifier.other doi: [10.11604/pamj.2014.19.128.4749]
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/25430
dc.description.abstract Introduction: Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection (ALRI) in young children. RSV strains have been divided into 2 major antigenic groups (A and B), which are further divided into several genotypes, but very little is known about its circulating genotypes in Ghana. This study characterized RSV genotypes detected in children with ALRI in Accra between February and November 2006.Methods: Nasopharyngeal aspirates (NPA) were obtained from children diagnosed with ALRI between February and November 2006. The NPA were screened for RSV using a nested multiplex reverse transcriptase polymerase chain reaction (RT-PCR) method for genotyping RSV. Viral RNA was extracted from the NPA using guanidinium isothiocyanate method and purified with an RNAID commercial kit. Care-givers gave their consent prior to specimen collection. Administered questionnaires captured information on patient demographic and clinical history.Results: A total of 53 children were enrolled in the study with a male to female ratio of 3:1. Of the 53 NPA analyzed, 60.4% (32/53) were positive for RSV. Subsequent genotypic analysis showed that 72% (23/32) of the 60.4% RSV infections were RSV B only and 28% (9/32) were co-infections of both RSV A and B. Children between the ages of 2 - 12 months were the most affected age group per an RSV infection rate of 37.5% (12/32). No significant difference was detected in the recovery rate of ALRI (98.1%) and RSV (96.9%) positive patients from the infection. One patient died resulting in a mortality rate of 3.1%. Bronchopneumonia (20 out of 32 cases) was the major diagnosis on admission. RSV infection was seasonal dependent, described by 2 peaks in October and April-May.Conclusion: Both RSV A and RSV B genotypes co-circulated during the study period with RSV B predominating. RSV may possibly be the main pathogen of lower respiratory tract illness during epidemics in the wet seasons. Genotyping by the multiplex RT-PCR is one of the first attempts at molecular diagnosis of RSV infection in Ghana. © Evangeline Obodai et al. en_US
dc.language.iso en en_US
dc.publisher Pan African Medical Journal en_US
dc.subject Lower respiratory tract infections en_US
dc.subject Multiplex RT-PCR en_US
dc.subject Respiratory syncytial viruses en_US
dc.title Respiratory syncytial virus genotypes circulating in urban Ghana: February to November 2006 en_US
dc.type Article en_US


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  • Virology Department [50]
    Most research activities involve the use of molecular methods such as regular PCR (PCR), quantitative PCR (Q-PCR), genomic sequence and analysis using different software, genetic engineering, probe hybridization techniques, biological and molecular cloning, evaluation of immune markers for laboratory diagnosis of infections, serological assays involving the use of rapid tests, ELISA-based evaluations and immunofluorescent assay techniques.

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