Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance

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dc.contributor.author Stratton, D.
dc.contributor.author Lange, S.
dc.contributor.author Kholia, S.
dc.contributor.author Jorfi, S.
dc.contributor.author Antwi-Baffour, S.
dc.contributor.author Inal, J.
dc.date.accessioned 2018-11-08T15:12:52Z
dc.date.available 2018-11-08T15:12:52Z
dc.date.issued 2014-10
dc.identifier.other https://doi.org/10.1016/j.bbrc.2014.09.132
dc.identifier.other Volume 453, Issue 3, Pages 619-624
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/25428
dc.description.abstract Using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D (label-free system) measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, we showed the attachment, over a 60 min period, of a monolayer of PC3 cells to the gold electrodes of the quartz crystal sensor, which had been rendered hydrophilic. That MVs were released upon BzATP stimulation of cells was confirmed by NTA analysis (average 250 nm diameter), flow cytometry, showing high phosphatidylserine exposition and by fluorescent (Annexin V Alexa Fluor® 488-positive) and electron microscopy. Over a period of 1000s (16.7 min) during which early apoptosis increased from 4% plateauing at 10% and late apoptosis rose to 2%, the Δf increased 20 Hz, thereupon remaining constant for the last 1000s of the experiment. Using the Sauerbrey equation, the loss in mass, which corresponded to the release of 2.36 × 106 MVs, was calculated to be 23 ng. We therefore estimated the mass of an MV to be 0.24 pg. With the deposition on the QCM-D of 3.5 × 107 MVs over 200s, the decrease in Δf (Hz) gave an estimate of 0.235 pg per MV. © 2014 Elsevier Inc. All rights reserved. en_US
dc.language.iso en en_US
dc.publisher Biochemical and Biophysical Research Communications en_US
dc.subject Microvesicles en_US
dc.subject Prostate cancer cells (PC3) en_US
dc.subject Quartz Crystal Microbalance en_US
dc.subject Real-time analysis en_US
dc.subject Stimulated release en_US
dc.title Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance en_US
dc.type Article en_US


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