dc.identifier.citation |
Ide T, Komoto S, Higo-Moriguchi K, Htun KW, Myint YY, Myat TW, et al. (2015) Whole Genomic Analysis of Human G12P[6] and G12P[8] Rotavirus Strains that Have Emerged in Myanmar. PLoS ONE 10(5): e0124965. |
en_US |
dc.description.abstract |
G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and
young children worldwide. However, the whole genomes of only a few G12 strains have
been fully sequenced and analyzed. In this study, we sequenced and characterized the
complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/
Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/
Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/
Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute
gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains
were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1
for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for
strain A23. Phylogenetic analysis showed that most genes of the six strains examined in
this study were genetically related to globally circulating human G1, G3, G9, and G12
strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with
the cognate genes of human-like bovine strains as well as human strains, suggesting the
occurrence of reassortment between human and bovine strains. Furthermore, strains A14,
A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments,
indicating derivation of the five strains from a common origin. On the other hand,
strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a
distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To ourknowledge, this is the first report on whole genome-based characterization of G12 strains
that have emerged in Myanmar. Our observations will provide important insights into the
evolutionary dynamics of spreading G12 rotaviruses in Asia. |
en_US |