Differences in innate cytokine responses between European and African children

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dc.contributor.author Labuda, L.A.
dc.contributor.author De Jong, S.E.
dc.contributor.author Meurs, L.
dc.contributor.author Amoah, A.S.
dc.contributor.author Mbow, M.
dc.contributor.author Ateba-Ngoa, U.
dc.contributor.author Van Der Ham, A.J.
dc.contributor.author Knulst, A.C.
dc.contributor.author Yazdanbakhsh, M.
dc.contributor.author Adegnika, A.A.
dc.date.accessioned 2017-10-26T12:07:46Z
dc.date.available 2017-10-26T12:07:46Z
dc.date.issued 2014
dc.identifier.other 10.1371/journal.pone.0095241
dc.identifier.uri http://ugspace.ug.edu.gh/handle/123456789/22259
dc.description.abstract Although differences in immunological responses between populations have been found in terms of vaccine efficacy, immune responses to infections and prevalence of chronic inflammatory diseases, the mechanisms responsible for these differences are not well understood. Therefore, innate cytokine responses mediated by various classes of patternrecognition receptors including Toll-like receptors (TLR), C-type lectin receptors (CLRs) and nucleotide-binding oligomerisation domain-like receptors (NLRs) were compared between Dutch (European), semi-urban and rural Gabonese (African) children. Whole blood was stimulated for 24 hours and the pro-inflammatory tumor necrosis factor (TNF) and the anti-inflammatory/regulatory interleukin-10 (IL-10) cytokines in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Gabonese children had a lower pro-inflammatory response to poly(I:C) (TLR3 ligand), but a higher pro-inflammatory response to FSL-1 (TLR2/6 ligand), Pam3 (TLR2/1 ligand) and LPS (TLR4 ligand) compared to Dutch children. Anti-inflammatory responses to Pam3 were also higher in Gabonese children. Non-TLR ligands did not induce substantial cytokine production on their own. Interaction between various TLR and non-TLR receptors was further assessed, but no differences were found between the three populations. In conclusion, using a field applicable assay, significant differences were observed in cytokine responses between European and African children to TLR ligands, but not to non-TLR ligands. en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.title Differences in innate cytokine responses between European and African children en_US
dc.type Article en_US


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  • Parasitology Department [241]
    The Department of Parasitology conducts research into parasitic diseases of public health importance with the overall goal of reducing their transmission and the heavy disease burden that they impose on affected populations. The Department maintains focus on parasitic diseases in general. These include major diseases such as malaria, and others listed under the Neglected Tropical Diseases (NTD) control initiative such as, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminthiasis, trypanosomiasis and leishmaniasis.

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