Chracterisation of the insulinotropic activity of aqueous extracts of Gymnema sylvestre in mouse B-cells and human Islets of Langerhans

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dc.contributor.author Anane-Asare, H.
dc.contributor.author Liu, B.
dc.contributor.author Al-Romaiyan, A.
dc.contributor.author Ameil, S.A.
dc.contributor.author Huang, G.C.
dc.contributor.author Jones, P.M.
dc.contributor.author Persaud, S.J.
dc.date.accessioned 2012-05-18T10:35:12Z
dc.date.accessioned 2017-10-19T11:50:47Z
dc.date.available 2012-05-18T10:35:12Z
dc.date.available 2017-10-19T11:50:47Z
dc.date.issued 2009
dc.identifier.citation Cell. Physio. Biochem (23): 125-132 en_US
dc.identifier.uri http://197.255.68.203/handle/123456789/1548
dc.description.abstract Leaves of the Gymnema sylvestre (GS) plant have been used to treat diabetes mellitus for millennia, but the previously documented insulin secretagogue effects of GS extracts in vitro may be non-physiological through damage to the beta-cells. We have now examined the effects of a novel GS extract (termed OSA) on insulin secretion from the MIN6 beta-cell line and isolated human islets of Langerhans. Insulin secretion from MIN6 cells was stimulated by OSA in a concentration-dependent manner, with low concentrations (0.06-0.25 mg/ml) having no deleterious effects on MIN6 cell viability, while higher concentrations (> or = 0.5 mg/ml) caused increased Trypan blue uptake. OSA increased beta-cell Ca2+ levels, an effect that was mediated by Ca2+ influx through voltage-operated calcium channels. OSA also reversibly stimulated insulin secretion from isolated human islets and its insulin secretagogue effects in MIN6 cells and human islets were partially dependent on the presence of extracellular Ca2+. These data indicate that low concentrations of the GS isolate OSA stimulate insulin secretion in vitro, at least in part as a consequence of Ca2+ influx, without compromising beta-cell viability. Identification of the component of the OSA extract that stimulates regulated insulin exocytosis, and further investigation of its mode(s) of action, may provide promising lead targets for Type 2 diabetes therapy. en_US
dc.language.iso en en_US
dc.publisher Cell. Physio. Biochem en_US
dc.title Chracterisation of the insulinotropic activity of aqueous extracts of Gymnema sylvestre in mouse B-cells and human Islets of Langerhans en_US
dc.type Article en_US


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